Department of Functional Anatomy, Graduate School of Medical Sciences, Kanazawa University, Takara-machi, Kanazawa, 920-8640, Japan; Department of Medical and Biological Physics, Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Partizan Zheleznyak st. 1, 660022, Krasnoyarsk, Russia.
Department of Functional Anatomy, Graduate School of Medical Sciences, Kanazawa University, Takara-machi, Kanazawa, 920-8640, Japan.
Biochem Biophys Res Commun. 2024 Nov 19;734:150625. doi: 10.1016/j.bbrc.2024.150625. Epub 2024 Aug 29.
Pain is a complex phenomenon that involves sensory, emotional, and cognitive components. The posterior insula cortex (pIC) has been shown to integrate multisensory experience with emotional and cognitive states. However, the involvement of the pIC in the regulation of affective behavior in pain remains unclear. Here, we investigate the role of pain-related pIC neurons in the regulation of anxiety-like behavior during acute pain. We combined a chemogenetic approach with targeted recombination in active populations (TRAP) in mice. Global chemogenetic inhibition of pIC neurons attenuates chemically-induced mechanical hypersensitivity without affecting pain-related anxiety-like behavior. In contrast, inhibition of pain-related pIC neurons reduces both mechanical hypersensitivity and pain-related anxiety-like behavior. The present study provides important insights into the role of pIC neurons in the regulation of sensory and affective pain-related behavior.
疼痛是一种复杂的现象，涉及感觉、情感和认知成分。后岛叶皮层（pIC）已被证明可以将多感觉体验与情绪和认知状态整合在一起。然而，pIC 在疼痛相关的情感行为调节中的作用尚不清楚。在这里，我们研究了与疼痛相关的 pIC 神经元在调节急性疼痛期间焦虑样行为中的作用。我们在小鼠中结合了化学遗传学方法和靶向活性群体重组（TRAP）。全局化学遗传抑制 pIC 神经元可减轻化学诱导的机械性超敏反应，而不影响与疼痛相关的焦虑样行为。相比之下，抑制与疼痛相关的 pIC 神经元可降低机械性超敏反应和与疼痛相关的焦虑样行为。本研究为 pIC 神经元在调节感觉和情感相关疼痛行为中的作用提供了重要的见解。
