Muneer Arooj, Akhtar Wasim, Samad Memoona, Zafar Sadia, Fatima Iram, Abidi Syed Hussain Imam, Kalsoom Razia, Shahbaz Amir
Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
Department of Botany, Division of Science and Technology, University of Education, Lahore, Punjab, Pakistan.
Microsc Res Tech. 2025 Jan;88(1):163-171. doi: 10.1002/jemt.24695. Epub 2024 Sep 5.
The green synthesis of silver nanoparticles (AgNPs) using plant-based derivatives is getting attention for biological applications because of their small dimensions and shape. In this study, AgNPs were prepared using leaf extract of Argyrolobium roseum (A. roseum) (Camb.) Jaub. & Spach. and then characterized via Fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), scanning electron microscopy (SEM) and ultraviolet-visible absorption (UV-Vis) spectroscopy. The UV-visible spectrum displayed a absorption peak at 450 nm and x-ray diffraction depicted a crystalline nature of biogenic NPs. FTIR analysis showed various functional groups involved in the reduction and capping of AgNPs while SEM revealed the spherical form of synthesized AgNPs. The antibacterial assay was conducted using disc diffusion assay and highest inhibition zones were recorded against Bacillus subtilis (B. subtilis) (9.6 ± 0.5 mm) and Staphylococcus aureus (S. aureus) (8.6 ± 0.5 mm). The antioxidant potential was assessed via DPPH scavenging assay and highest percentage inhibition (89%) was observed at 100 μg/mL. Subsequently, different concentrations of A. roseum AgNPs were applied on the wheat seedlings to investigate its effects on different growth parameters. After applying AgNPs, significant increase in the fresh weight (FW), dry weight (DW), root length (RL), shoot length (SL), leaf number (LN) and chlorophyll content (CC) in wheat (Akbar-2019 variety) seedlings was observed in comparison to the control seedlings. Overall, A. roseum mediated synthesis of AgNPs was cost-effective and safe and can be used in agriculture, biomedical and other fields. RESEARCH HIGHLIGHTS: Synthesis and characterization of A. roseum AgNPs was done. Biogenic AgNPs revealed potent antibacterial and antioxidant potential. A. roseum mediated AgNPs also increases the growth and germination of wheat seedlings.
利用植物衍生材料绿色合成银纳米颗粒（AgNPs）因其尺寸小和形状而在生物应用方面受到关注。在本研究中，使用玫瑰叶豆（Argyrolobium roseum (A. roseum) (Camb.) Jaub. & Spach.）的叶提取物制备了AgNPs，然后通过傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）和紫外可见吸收光谱（UV-Vis）对其进行了表征。紫外可见光谱在450nm处显示出一个吸收峰，X射线衍射表明生物源纳米颗粒具有晶体性质。FTIR分析显示了参与AgNPs还原和包覆的各种官能团，而SEM揭示了合成的AgNPs的球形形态。采用纸片扩散法进行抗菌试验，对枯草芽孢杆菌（B. subtilis）（9.6±0.5mm）和金黄色葡萄球菌（S. aureus）（8.6±0.5mm）记录到最高抑菌圈。通过DPPH清除试验评估抗氧化潜力，在100μg/mL时观察到最高抑制率（89%）。随后，将不同浓度的玫瑰叶豆AgNPs施用于小麦幼苗，以研究其对不同生长参数的影响。与对照幼苗相比，施用AgNPs后，小麦（Akbar-2019品种）幼苗的鲜重（FW）、干重（DW）、根长（RL）、茎长（SL）、叶片数（LN）和叶绿素含量（CC）显著增加。总体而言，玫瑰叶豆介导的AgNPs合成具有成本效益且安全，可用于农业、生物医学和其他领域。研究亮点：完成了玫瑰叶豆AgNPs的合成与表征。生物源AgNPs显示出强大的抗菌和抗氧化潜力。玫瑰叶豆介导的AgNPs还能促进小麦幼苗的生长和萌发。
