Meletis J, Yataganas X, Eliopoulos G, Panourgias J, Loukopoulos D, Fessas P
Acta Haematol. 1985;73(1):16-21. doi: 10.1159/000206266.
The hemoglobin content of single erythrocytes was determined by microspectrophotometry as total extinction (TE) at 415 nm in subjects with a wide spectrum of hemoglobin levels and erythrocyte indices. Precise measurements of the cellular area (A) and the ratio TE/A were also recorded. A significant correlation was found between TE and mean corpuscular hemoglobin (MCH), A and mean corpuscular volume, and TE/A and mean cell hemoglobin concentration (MCHC) for the cases studied. In addition, TE, A and TE/A were determined in fetal erythrocytes obtained by fetoscopy at the 20th week of gestation for prenatal diagnosis of beta-thalassemia. The mean red cell TE and TE/A of the group of fetuses diagnosed to have thalassemia major were significantly lower than those of the group of normal controls and with heterozygous beta-thalassemia. The significant differences of TE, A and TE/A between maternal and fetal blood allowed a safe distinction of the latter. The calculated MCH of the fetuses with thalassemia major was 12% less than that of the normal controls; the respective difference of the mean cell size was 5% and that of MCHC 3% lower than normal.
采用显微分光光度法，在具有广泛血红蛋白水平和红细胞指数的受试者中，测定单个红细胞的血红蛋白含量，以415nm处的总消光值（TE）表示。同时还精确测量了细胞面积（A）以及TE/A比值。在所研究的病例中，发现TE与平均红细胞血红蛋白含量（MCH）、A与平均红细胞体积、TE/A与平均细胞血红蛋白浓度（MCHC）之间存在显著相关性。此外，在妊娠20周时通过胎儿镜检查获取胎儿红细胞，测定TE、A和TE/A，用于β地中海贫血的产前诊断。诊断为重型地中海贫血的胎儿组的平均红细胞TE和TE/A显著低于正常对照组和杂合子β地中海贫血组。母体血与胎儿血之间TE、A和TE/A的显著差异使得能够安全地区分胎儿血。重型地中海贫血胎儿的计算MCH比正常对照组低12%；平均细胞大小的相应差异为5%，MCHC比正常低3%。
