School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255000, P. R. China.
School of Medical Laboratory, Qilu Medical University, Zibo 255000, P. R. China.
J Agric Food Chem. 2024 Sep 18;72(37):20615-20621. doi: 10.1021/acs.jafc.4c05893. Epub 2024 Sep 6.
Pesticide residues pose a significant threat to food safety and human health, necessitating the development of novel detection tools. Pesticides can inhibit the activity of certain biological enzymes, so enzyme inhibition is one of the methods of pesticide detection. In this study, we developed a novel near-infrared fluorescent probe named based on the tricyanofuran structure, for ultrasensitive detection of carboxylesterase (CES). exhibits strong and stable fluorescence, excellent specificity. Notably, the fluorescence intensity of shows a linear relationship with CES concentration, achieving an exceptionally low detection limit of 4.41 × 10 u/mL. This ultrasensitive probe can also effectively detect pesticide residues in vegetables and monitor CES activity in cells and liver tissues. stands out for its rapid and accurate detection capabilities, making it an essential tool for accurately monitoring pesticide residue. It also has great potential for tracking CES activity in biological systems. Additionally, it offers a robust solution for food safety and improving pesticide residue analysis.
农药残留对食品安全和人类健康构成重大威胁，因此需要开发新型检测工具。农药可以抑制某些生物酶的活性，因此酶抑制是农药检测的方法之一。在本研究中，我们基于三嗪呋喃结构开发了一种新型近红外荧光探针，用于超灵敏检测羧酸酯酶 (CES)。 表现出强而稳定的荧光，具有优异的特异性。值得注意的是， 的荧光强度与 CES 浓度呈线性关系，检测限低至 4.41×10 u/mL。该超灵敏探针还可以有效检测蔬菜中的农药残留，并监测细胞和肝组织中的 CES 活性。 因其快速准确的检测能力而脱颖而出，是准确监测农药残留的重要工具。它在跟踪生物系统中的 CES 活性方面也具有巨大的潜力。此外，它为食品安全和改善农药残留分析提供了可靠的解决方案。
