Feeley T W, Mihm F G, Downing T P, Sadeghi A M, Baumgartner W A, Reitz B A, Shumway N E
Ann Thorac Surg. 1985 Jun;39(6):558-62. doi: 10.1016/s0003-4975(10)61998-4.
The effect of hypothermic preservation of the heart and lungs with a crystalloid solution was evaluated in 12 mongrel dogs receiving heart-lung allografts. Six animals served as controls and received an immediate heart-lung transplant. Six animals were in the experimental group and received a heart-lung transplant after 5 hours of preservation at 4 degrees C following perfusion of both organs with a crystalloid solution. Physiological function of the heart and lungs was studied for 20 hours after transplantation. While cardiac function was minimally depressed following preservation, pulmonary function testing demonstrated significantly greater increases in extravascular lung water in experimental animals, suggesting that an ischemic lung injury occurred with this preservation technique. The model allows for future evaluation of other methods of combined preservation of both the heart and lungs for transplantation.
在12只接受心肺同种异体移植的杂种犬中，评估了用晶体溶液低温保存心肺的效果。6只动物作为对照，接受即刻心肺移植。6只动物在实验组，在用晶体溶液灌注两个器官后，于4℃保存5小时后接受心肺移植。移植后对心肺的生理功能进行了20小时的研究。虽然保存后心脏功能仅有轻微下降，但肺功能测试表明，实验动物的血管外肺水显著增加，这表明这种保存技术会导致缺血性肺损伤。该模型有助于未来评估用于移植的心肺联合保存的其他方法。
