Department of Biology, College of Science, Mustanceryia University, Baghdad, Iraq.
Department of Physiology, High Institute of Infertility Diagnosis and Art, Al-Nahrain University, Baghdad, Iraq.
Hum Antibodies. 2024;32(4):221-227. doi: 10.3233/HAB-240032.
Infertility is an inability to conceive after a reasonable period of time (12 months) without the use of contraception or due to a person's incapacity to procreate, whether independently or with a spouse. Problems with the production and maturation of sperm are the most common causes of male infertility additionally; the motility is the major functional character that determines the fertilizing ability of spermatozoa. Therefore the goal of this study is to get better certain sperm function parameters in vitro of asthenozoospermic patient.
The World Health Organization (WHO) and many studies considered the infertility as a disease and so many couples complaining from unsuccessful assisted reproductive technologies (ART) procedures to overcome their problem. The goal of this study is to improve certain sperm function feature in vitro of asthenozoospermic semen patients by using combination of motility inducing namely; Maca, L-carnitine and Pentoxifylline that enhance the medium to improve certain sperm characters that might be utilized for ART centers.
Semen aliquots were collected from ninety patients with asthenozoospermia who participated in present study, the volume of semen samples with normal ejaculate when was ranged between 1.4-6ml and can be measured by using a measure pipette or conical graduated tube; Inclusion criteria was Asthenozoospermia, oligozoospermia and teratozoospermia men, Infertile idiopathic men also, fertile normozoospermic men. While Exclusion criteria was Azoospermic men, Alcoholic, Patients under treatment with antibiotics and men with Varicocele.The samples split into two equal groups at random. Using Ham's F12 medium, one portion served as the control group, and the other was the treatment group, which was mixing by combining the following ingredients, Maca powder extracts (Lepidium meyenii) (M) 1 mg/ml, 0.5 mg/ml of L-Carnitine (LC), and 10 mg/ml of Pentoxifylline (PTX). The data were analyzed using Statistical Package for Social Sciences (SPSS) version 23.0. The descriptive statistics including frequency, range, mean and standard error, Data from treated and control groups were expressed as mean ± SEM and to compare value between experimental and control groups using Students t-test. Layering approach is used to investigate sperm parameters before and after in vitro activation.
The information showed a very large (p< 0.001) increase in active sperm motility grade A, percentage of progressive motility with significant increase in morphologically normal sperm (MNS) with decreased in DNA fragmentation index after activation by layering technique with novel combination medium compared to Hams F12 medium and before activation.
The present work stated that novel combination medium (LC, maca and PXT) have potential effects to improve sperm characters in male infertility factors and suggested to be used for sperm preparation and activation in ART programs.
不孕是指在没有使用避孕措施或由于个人生育能力问题而无法在合理时间（12 个月）内怀孕。此外，精子的产生和成熟问题是导致男性不育的最常见原因；精子的活力是决定精子受精能力的主要功能特征。因此，本研究的目的是改善弱精症患者的某些体外精子功能参数。
世界卫生组织（WHO）和许多研究将不孕视为一种疾病，因此许多夫妇因辅助生殖技术（ART）程序不成功而抱怨，无法解决他们的问题。本研究的目的是通过使用运动诱导剂，即玛卡、左旋肉碱和己酮可可碱的组合，来改善弱精症精液患者的某些精子功能特征，从而提高精子的某些特征，这些特征可能用于 ART 中心。
从 90 名患有弱精症的患者中收集精液样本，这些患者参与了本研究，正常射精的精液量在 1.4-6ml 之间，可以使用测量吸管或锥形刻度管进行测量；纳入标准为弱精症、少精症和畸形精子症男性，特发性不孕男性，正常生育能力的正常精子症男性。而排除标准为无精症男性、酗酒者、正在接受抗生素治疗的患者和精索静脉曲张患者。将样本随机分成两组。使用 Ham's F12 培养基，一部分作为对照组，另一部分作为实验组，实验组通过混合以下成分进行混合：玛卡粉提取物（Lepidium meyenii）（M）1mg/ml、0.5mg/ml 的左旋肉碱（LC）和 10mg/ml 的己酮可可碱（PTX）。使用社会科学统计软件包（SPSS）版本 23.0 分析数据。描述性统计包括频率、范围、平均值和标准误差，处理组和对照组的数据表示为平均值±SEM，使用学生 t 检验比较实验组和对照组之间的值。分层方法用于在体外激活前后研究精子参数。
结果表明，与 Ham's F12 培养基和激活前相比，新型组合培养基（LC、玛卡和 PXT）通过分层技术激活后，精子活力 A 级、前向运动精子百分比和形态正常精子（MNS）显著增加，DNA 碎片指数显著降低。
本研究表明，新型组合培养基（LC、玛卡和 PXT）具有改善男性不育因素中精子特征的潜在作用，并建议将其用于 ART 项目中的精子准备和激活。
