Clark Beth Z, Soong T Rinda, Goel Kanika, Elishaev Esther, Zhao Chengquan, Jones Terri E, Jones Mirka W, Skvarca Lauren B, Motanagh Samaneh A, Carter Gloria J, Fine Jeffrey L, Harinath Lakshmi, Villatoro Tatiana M, Yu Jing, Bhargava Rohit
Department of Pathology, University of Pittsburgh School of Medicine, UPMC Magee-Womens Hospital, Pittsburgh, PA, US.
Am J Clin Pathol. 2025 Jan 28;163(1):143-152. doi: 10.1093/ajcp/aqae104.
The objective of this study was to evaluate SOX17, a transcription factor from the Sry high-mobility group-related box superfamily, as a diagnostic marker to determine site of origin using both whole-tissue sections and tissue microarrays (TMAs).
SOX17 immunohistochemistry was performed on gynecologic and nongynecologic tissues (N = 1004) using whole-tissue sections and both internally constructed and commercially available TMAs. SOX17 nuclear reactivity was scored as positive or negative on the whole-tissue sections and using the semiquantitative H score method on TMAs.
Using both whole-tissue sections and TMAs, SOX17 was positive in 94% (n = 155) of endometrial tumors and 96% (n = 242) of ovarian tumors. All breast cases (n = 241) and vulvar/cervical squamous cell carcinomas (n = 150) were negative. Among 1004 tumors from 20 sites, the only organs with positive tumors were ovary, uterus, and testis.
SOX17 is a sensitive and specific marker for gynecologic origin in the tissues tested and may be a valuable adjunct to PAX8 and other commonly used markers to confirm endometrial or ovarian origin. SOX17 expression is lower in mucinous tumors, endocervical adenocarcinoma, high-grade neuroendocrine tumors, and undifferentiated/dedifferentiated endometrial carcinoma.
本研究的目的是评估SOX17，一种来自Sry高迁移率族相关盒超家族的转录因子，作为一种诊断标志物，通过全组织切片和组织微阵列（TMA）来确定肿瘤的起源部位。
使用全组织切片以及内部构建和市售的TMA，对妇科和非妇科组织（N = 1004）进行SOX17免疫组织化学检测。在全组织切片上，将SOX17核反应性评为阳性或阴性，并在TMA上使用半定量H评分法。
使用全组织切片和TMA，SOX17在94%（n = 155）的子宫内膜肿瘤和96%（n = 242）的卵巢肿瘤中呈阳性。所有乳腺癌病例（n = 241）和外阴/宫颈鳞状细胞癌（n = 150）均为阴性。在来自20个部位的1004个肿瘤中，肿瘤呈阳性的唯一器官是卵巢、子宫和睾丸。
在测试的组织中，SOX17是妇科起源的敏感和特异性标志物，可能是PAX8和其他常用标志物的有价值辅助指标，用于确认子宫内膜或卵巢起源。在黏液性肿瘤、宫颈管腺癌、高级别神经内分泌肿瘤和未分化/去分化子宫内膜癌中，SOX17表达较低。
