School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
J Chromatogr A. 2024 Oct 25;1735:465313. doi: 10.1016/j.chroma.2024.465313. Epub 2024 Aug 31.
In this study, we synthesized and employed an ionic gel-functionalized silica stationary phase for high-performance liquid chromatography. The successful fabrication of the stationary phase was confirmed through attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), zeta-potential measurements, and elemental analysis (EA). Comparative performance evaluation against a commercial column demonstrated the prepared column's effectiveness in the mixed mode of reversed-phase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), and ion chromatography (IC). Moreover, the stationary phase exhibited exceptional retention repeatability in per aqueous liquid chromatography, showcasing its potential as an environmentally friendly analytical method. Mechanistic investigations unveiled multiple solute-stationary phase interactions, including π-π interactions, hydrogen bonding, and ion exchange. Finally, we applied the developed stationary phase for the precise detection of preservatives in carbonated beverages and jelly, achieving high levels of accuracy and recovery rates.
在这项研究中，我们合成并使用了一种离子凝胶功能化硅胶固定相用于高效液相色谱。固定相的成功制备通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、X 射线光电子能谱（XPS）、动电位测量和元素分析（EA）得到了确认。与商业柱的比较性能评估表明，制备的柱子在反相液相色谱（RPLC）、亲水相互作用液相色谱（HILIC）和离子色谱（IC）的混合模式下有效。此外，该固定相在水相液相色谱中表现出出色的保留重复性，展示了其作为一种环境友好的分析方法的潜力。机理研究揭示了多种溶质-固定相相互作用，包括π-π 相互作用、氢键和离子交换。最后，我们将开发的固定相应用于碳酸饮料和果冻中防腐剂的精确检测，实现了高准确度和高回收率。
