Linder William Emory, Clark Wendy Auclair, Arnold Roland, De Kok Ingeborg, Felton David A
Private practice, Charlotte, NC.
Associate Professor, Prosthodontics, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC.
J Prosthet Dent. 2024 Sep 5. doi: 10.1016/j.prosdent.2024.07.040.
Though computer-aided design and computer-aided manufactured (CAD-CAM) denture bases have become popular, evidence on the ability of C. albicans cells to adhere to these denture bases is lacking.
The purpose of this in vitro study was to evaluate the adherence of Candida albicans to differently manufactured acrylic resin denture bases.
Acrylic resin disks were fabricated using a total of 6 different fabrication methods (compression molding, injection molding, CAD-CAM milling, and rapid prototyping on 3 different printers with 3 different resins). Each material was evaluated for adherence of C. albicans using 2 different experimental methods - suspension in inoculated tryptic soy broth (TSB) or placed onto a uniform lawn of C. albicans on tryptone soya agar (TSA) with 5% sheep's blood. Attached cells were quantified by spiral plating and then used to re-inoculate sterile plates. Logarithmic transformation was completed to normalize data. For the broth suspension, the Kruskal-Wallis test was used to identify any differences between the 6 specimen types in terms of recovery, and the Dunn test was used for post hoc analysis. For the microbial lawn experiment, 1-way analysis of variance (ANOVA) and then the Tukey Honestly Significant Difference (HSD) post hoc test were used.
Statistically significant differences were found between the numbers of adherent cells based on manufacturing method and between experimental designs (P<.05). All resins demonstrated growth with re-inoculation.
Though statistical significance was noted, neither experimental technique demonstrated what is likely a clinically significant preferential binding to any particular resin surface. Attached Candida cells are effective carriers of pathogens to uninfected surfaces. Further studies are indicated for potential virulence factors and differences in printed resins.
尽管计算机辅助设计和计算机辅助制造（CAD-CAM）的义齿基托已变得流行，但关于白色念珠菌细胞粘附于这些义齿基托能力的证据仍然缺乏。
本体外研究的目的是评估白色念珠菌对不同制造方法的丙烯酸树脂义齿基托的粘附情况。
使用总共6种不同的制造方法（压缩成型、注射成型、CAD-CAM铣削以及在3台不同打印机上使用3种不同树脂进行快速成型）制作丙烯酸树脂圆盘。每种材料使用2种不同的实验方法评估白色念珠菌的粘附情况——接种于胰蛋白胨大豆肉汤（TSB）中悬浮或置于含5%羊血的胰蛋白胨大豆琼脂（TSA）上的白色念珠菌均匀菌苔上。通过螺旋平板接种法定量附着的细胞，然后用于重新接种无菌平板。进行对数转换以标准化数据。对于肉汤悬浮实验，使用Kruskal-Wallis检验来确定6种样本类型在回收率方面的任何差异，并使用Dunn检验进行事后分析。对于菌苔实验，使用单因素方差分析（ANOVA），然后使用Tukey真实显著差异（HSD）事后检验。
基于制造方法和实验设计，在粘附细胞数量之间发现了统计学上的显著差异（P<0.05）。所有树脂重新接种后均显示生长。
尽管注意到了统计学显著性，但两种实验技术均未证明对任何特定树脂表面存在可能具有临床意义的优先结合。附着的念珠菌细胞是病原体向未感染表面的有效载体。需要进一步研究潜在的毒力因子以及打印树脂的差异。
