Aslanimehr Masoomeh, Rezvani Shirin, Mahmoudi Ali, Moosavi Najmeh
Dept. of Microbiology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
J Dent (Shiraz). 2017 Mar;18(1):61-64.
Candida species are believed to play an important role in initiation and progression of denture stomatitis. The type of the denture material also influences the adhesion of candida and development of stomatitis.
The aim of this study was comparing the adherence of to the conventional and injection molding acrylic denture base materials.
Twenty injection molding and 20 conventional pressure pack acrylic discs (10×10×2 mm) were prepared according to their manufacturer's instructions. Immediately before the study, samples were placed in sterile water for 3 days to remove residual monomers. The samples were then sterilized using an ultraviolet light unit for 10 minutes. 1×10 Cfu/ml suspension of ATCC-10231 was prepared from 48 h cultured organism on sabouraud dextrose agar plates incubated at 37oC. 100 μL of this suspension was placed on the surface of each disk. After being incubated at 37oC for 1 hour, the samples were washed with normal saline to remove non-adherent cells. Attached cells were counted using the colony count method after shaking at 3000 rmp for 20 seconds. Finally, each group was tested for 108 times and the data were statistically analyzed by t-test.
Quantitative analysis revealed that differences in colony count average of adherence to conventional acrylic materials (8.3×10) comparing to injection molding acrylic resins (6×10) were statistically significant (<0.001).
Significant reduction of adherence to the injection acrylic resin materials makes them valuable for patients with high risk of denture stomatitis.
假丝酵母菌属被认为在义齿性口炎的发生和发展中起重要作用。义齿材料的类型也会影响念珠菌的黏附及口炎的发展。
本研究的目的是比较[具体念珠菌名称未给出]对传统和注塑成型丙烯酸义齿基托材料的黏附情况。
按照制造商说明制备20个注塑成型和20个传统压力包丙烯酸圆盘（10×10×2毫米）。在研究前，将样品立即置于无菌水中3天以去除残留单体。然后使用紫外线装置对样品进行10分钟消毒。从在37℃孵育48小时的沙氏葡萄糖琼脂平板上培养的生物体中制备1×10菌落形成单位/毫升的[具体念珠菌名称未给出]ATCC - 10231悬浮液。将100微升此悬浮液置于每个圆盘表面。在37℃孵育1小时后，用生理盐水洗涤样品以去除未黏附的细胞。在以3000转/分钟振荡20秒后，使用菌落计数法对黏附细胞进行计数。最后，每组进行108次测试，并通过t检验对数据进行统计分析。
定量分析显示，与注塑成型丙烯酸树脂（6×10）相比，[具体念珠菌名称未给出]对传统丙烯酸材料的黏附菌落计数平均值差异具有统计学意义（<0.001）（传统丙烯酸材料黏附菌落计数平均值为8.3×10）。
[具体念珠菌名称未给出]对注塑丙烯酸树脂材料的黏附显著降低，这使其对义齿性口炎高风险患者具有价值。
