Long Lin-Zi, Tan Ling, Xu Feng-Qin, Yang Wen-Wen, Li Hong-Zheng, Liu Jian-Gang, Wang Ke, Zhao Zhi-Ru, Wang Yue-Qi, Wang Chao-Ju, Wen Yi-Chao, Huang Ming-Yan, Qu Hua, Fu Chang-Geng, Chen Ke-Ji
Department of Geriatrics, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing, 100091, China.
Department of Traditional Chinese Medicine, the Eighth Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province, 518033, China.
Chin J Integr Med. 2025 May;31(5):402-411. doi: 10.1007/s11655-024-3807-4. Epub 2024 Sep 7.
To assess the efficacy of Qingda Granule (QDG) in ameliorating hypertension-induced cardiac damage and investigate the underlying mechanisms involved.
Twenty spontaneously hypertensive rats (SHRs) were used to develope a hypertension-induced cardiac damage model. Another 10 Wistar Kyoto (WKY) rats were used as normotension group. Rats were administrated intragastrically QDG [0.9 g/(kg•d)] or an equivalent volume of pure water for 8 weeks. Blood pressure, histopathological changes, cardiac function, levels of oxidative stress and inflammatory response markers were measured. Furthermore, to gain insights into the potential mechanisms underlying the protective effects of QDG against hypertension-induced cardiac injury, a network pharmacology study was conducted. Predicted results were validated by Western blot, radioimmunoassay immunohistochemistry and quantitative polymerase chain reaction, respectively.
The administration of QDG resulted in a significant decrease in blood pressure levels in SHRs (P<0.01). Histological examinations, including hematoxylin-eosin staining and Masson trichrome staining revealed that QDG effectively attenuated hypertension-induced cardiac damage. Furthermore, echocardiography demonstrated that QDG improved hypertension-associated cardiac dysfunction. Enzyme-linked immunosorbent assay and colorimetric method indicated that QDG significantly reduced oxidative stress and inflammatory response levels in both myocardial tissue and serum (P<0.01).
Both network pharmacology and experimental investigations confirmed that QDG exerted its beneficial effects in decreasing hypertension-induced cardiac damage by regulating the angiotensin converting enzyme (ACE)/angiotensin II (Ang II)/Ang II receptor type 1 axis and ACE/Ang II/Ang II receptor type 2 axis.
评估清大颗粒（QDG）改善高血压所致心脏损伤的疗效，并探究其潜在机制。
选用20只自发性高血压大鼠（SHRs）建立高血压所致心脏损伤模型。另选10只Wistar Kyoto（WKY）大鼠作为正常血压组。大鼠灌胃给予QDG[0.9 g/(kg•d)]或等体积纯水，持续8周。测量血压、组织病理学变化、心脏功能、氧化应激水平及炎症反应标志物。此外，为深入了解QDG对高血压所致心脏损伤保护作用的潜在机制，进行了网络药理学研究。预测结果分别通过蛋白质免疫印迹法、放射免疫分析法、免疫组织化学法和定量聚合酶链反应进行验证。
给予QDG可使SHRs血压水平显著降低（P<0.01）。苏木精-伊红染色和Masson三色染色等组织学检查显示，QDG可有效减轻高血压所致心脏损伤。此外，超声心动图显示QDG改善了与高血压相关的心脏功能障碍。酶联免疫吸附测定法和比色法表明，QDG显著降低了心肌组织和血清中的氧化应激和炎症反应水平（P<0.01）。
网络药理学和实验研究均证实，QDG通过调节血管紧张素转换酶（ACE）/血管紧张素II（Ang II）/1型Ang II受体轴和ACE/Ang II/2型Ang II受体轴，在减轻高血压所致心脏损伤方面发挥有益作用。
