Microplastics in riverine systems: Recommendations for standardized sampling, separation, digestion and characterization.

Microplastic (MP) pollution has emerged as a global concern, prompting numerous studies on MP detection. Due to the remaining methodological challenges, it affects the accuracy and reliability of MP's impact assessment on river systems. To address this, the establishment of standardized operating protocols is crucial, encompassing sampling, separation, digestion, and characterization methods. This study evaluates the current tools used for identifying and quantifying MPs in riverine ecosystems, aiming to offer harmonized guidelines for future protocols. Recommendations include adopting a consistent format for reporting MP concentrations and providing improved information on sampling, separation, and digestion for enhanced cross-study comparisons. The importance of quality assurance and quality control is also discussed. Furthermore, we highlight unresolved issues, proposing avenues for further investigation. Suggestions encompass standardizing river sampling methods, optimizing technical steps and analysis processes, and enhancing the accuracy, reliability, and comparability of detection data to advance our understanding of MPs in river environments.