College of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510000, China.
Anal Chim Acta. 2024 Oct 9;1325:343115. doi: 10.1016/j.aca.2024.343115. Epub 2024 Aug 15.
Excessive use of veterinary drugs causes severely environmental pollution and agricultural pollution, and poses great threat to human health. A simple method for the rapid, highly sensitive, and on-site monitoring of veterinary drug residues in complex samples remains lacking.
In this study, we propose a catalytically enhanced colorimetric lateral flow immunoassay (LFA) based on a novel core-satellite-structured magnetic nanozyme (Fe-Au@Pt) that can simultaneously and quantitatively detect three common veterinary drugs, namely, gentamicin (GM), streptomycin (STR), and clenbuterol (CLE), within a short testing time (<30 min). The Fe-Au@Pt nanozyme was simply prepared through the self-assembly of numerous Au@Pt nanoparticles on a large FeO core via electrostatic adhesion, which exhibited the advantages of high peroxidase-like activity, strong magnetic responsiveness, and multiple catalytic sites. Under the dual-signal amplification effect of magnetic enrichment and catalytic enhancement, the proposed nanozyme-LFA allowed the multiplex detection of STR, CLE, and GM with detection limits of 10.1, 6.3, and 1.1 pg/mL, respectively.
The developed Fe-Au@Pt-LFA achieves direct, simultaneous, and accurate detection of three target drugs in food samples (honey, milk, and pork). The proposed assay shows great potential for application in the real-time monitoring of small-molecule pollutants in complex environment.
兽用药物的过度使用会造成严重的环境污染和农业污染，对人类健康构成极大威胁。目前仍缺乏一种用于快速、高灵敏、现场监测复杂样品中兽药残留的简单方法。
本研究提出了一种基于新型核-卫星结构磁性纳米酶（Fe-Au@Pt）的催化增强比色侧向流免疫分析（LFA），可在短时间（<30 分钟）内同时定量检测三种常见的兽药，即庆大霉素（GM）、链霉素（STR）和克伦特罗（CLE）。通过静电吸附将大量 Au@Pt 纳米颗粒自组装到大的 FeO 核上，简单制备了 Fe-Au@Pt 纳米酶，其具有过氧化物酶样活性高、磁响应性强、催化位点多等优点。在磁富集和催化增强的双重信号放大作用下，所提出的纳米酶-LFA 允许对 STR、CLE 和 GM 进行多重检测，检测限分别为 10.1、6.3 和 1.1 pg/mL。
所开发的 Fe-Au@Pt-LFA 可直接、同时、准确地检测食品样品（蜂蜜、牛奶和猪肉）中的三种目标药物。该测定法有望在实时监测复杂环境中的小分子污染物方面得到应用。
