Lu Wenjing, Chen Yuan, Ramírez María Daniela Artigas, Liu Yuning, Zhang Haolin, Yuan Zhengrong, Han Yingying, Weng Qiang
College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China.
College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China.
Comp Biochem Physiol B Biochem Mol Biol. 2025 Jan;275:111030. doi: 10.1016/j.cbpb.2024.111030. Epub 2024 Sep 7.
Mitochondria play a critical role in follicular development and ovulation, at least in part through the actions of growth hormone (GH)/insulin-like growth factor-1 (IGF-1) on mitochondrial biogenesis. This study aimed to identify seasonal alterations in the GH/IGF-1 system and mitochondrial biogenesis in muskrat (Ondatra zibethicus) ovaries. We utilized the muskrat, a typical seasonal breeder, to clarify the potential impact of the GH/IGF-1 system on mitochondrial biogenesis across different breeding seasons using immunohistochemistry, gene expression and high-throughput sequencing. Alterations in follicular development existed in muskrat ovaries between the breeding season (BS) and non-breeding season (NBS), accompanied by a striking decrease in circulating and ovarian GH and IGF-1 concentrations. GH, GHR, IGF-1, IGF-1R, and mitochondrial biogenesis markers were localized in the ovarian cells of muskrats during both seasons. In contrast, Gh, Ghr, Igf-1, Igf-1r, Ppargc1a, Ppargc1b, Tfam, and Nrf1/2 mRNA levels were higher in BS. The relative levels of GH and IGF-1 in circulation and ovaries were positively associated with mitochondrial biogenesis markers. Additionally, RNA-seq analysis demonstrated that differentially expressed genes might be associated with insulin and PI3K/Akt signaling pathways, as well as mitochondrial function-related pathways. These findings suggest that the intra-ovarian GH/IGF-1 system, which is associated with seasonal changes in mitochondrial biogenesis, is activated in muskrat ovaries in BS.
线粒体在卵泡发育和排卵过程中发挥着关键作用，至少部分是通过生长激素（GH）/胰岛素样生长因子-1（IGF-1）对线粒体生物发生的作用来实现的。本研究旨在确定麝鼠（麝鼠属）卵巢中GH/IGF-1系统和线粒体生物发生的季节性变化。我们利用麝鼠这一典型的季节性繁殖动物，通过免疫组织化学、基因表达和高通量测序来阐明GH/IGF-1系统在不同繁殖季节对线粒体生物发生的潜在影响。在繁殖季节（BS）和非繁殖季节（NBS）之间，麝鼠卵巢中的卵泡发育存在差异，同时循环和卵巢中的GH和IGF-1浓度显著降低。在两个季节中，GH、GHR、IGF-1、IGF-1R和线粒体生物发生标志物均定位于麝鼠的卵巢细胞中。相比之下，Gh、Ghr、Igf-1、Igf-1r、Ppargc1a、Ppargc1b、Tfam和Nrf1/2 mRNA水平在繁殖季节较高。循环和卵巢中GH和IGF-1的相对水平与线粒体生物发生标志物呈正相关。此外，RNA测序分析表明，差异表达基因可能与胰岛素和PI3K/Akt信号通路以及线粒体功能相关通路有关。这些发现表明，与线粒体生物发生的季节性变化相关的卵巢内GH/IGF-1系统在繁殖季节的麝鼠卵巢中被激活。
