Human Metabolomics, North-West University, Potchefstroom, South Africa.
DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, SAMRC Centre for TB Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa.
J Microbiol Methods. 2024 Nov;226:107028. doi: 10.1016/j.mimet.2024.107028. Epub 2024 Sep 6.
The use of detergents when culturing Mycobacterium tuberculosis (M. tuberculosis) are essential to prevent clumping. However, these detergents may influence research outcomes by impacting bacterial morphology and metabolism. This study aimed to assess the metabolome of a M. tuberculosis H37Rv strain cultured with Tyloxapol (H37Rv), compared to a control group of H37Rv strain cultured without detergent (H37Rv) to evaluate Tyloxapol's suitability for metabolomic studies. Distinct metabolic alterations were observed in H37Rv compared to H37Rv, primarily associated with fatty acid, sugar and pentose phosphate metabolic pathways. These changes are associated with the surface stress exerted by Tyloxapol on the bacteria, prompting an adaptation of M. tuberculosis metabolism to that usually observed in stress environments. Nevertheless, the effect of Tyloxapol is less pronounced than that of a previous investigation using Tween 80, indicating its potential as the more favourable choice for culturing M. tuberculosis for metabolomic analysis, with due consideration to dosage and result interpretation.
在培养结核分枝杆菌（M. tuberculosis）时使用清洁剂是防止结块的必要条件。然而，这些清洁剂可能会通过影响细菌形态和代谢来影响研究结果。本研究旨在评估与未用清洁剂（H37Rv）培养的 H37Rv 对照相比，用 Tyloxapol（H37Rv）培养的 M. tuberculosis H37Rv 株的代谢组，以评估 Tyloxapol 用于代谢组学研究的适宜性。与 H37Rv 相比，H37Rv 观察到明显的代谢改变，主要与脂肪酸、糖和戊糖磷酸代谢途径有关。这些变化与 Tyloxapol 对细菌表面施加的表面压力有关，促使 M. tuberculosis 代谢适应通常在应激环境中观察到的代谢。然而，Tyloxapol 的作用不如以前使用 Tween 80 的研究明显，表明其作为培养 M. tuberculosis 进行代谢组学分析的更有利选择，需考虑剂量和结果解释。
