Zhang Shixi, Zhang Yuhan, Jiang Jiaye, Charconnet Mathias, Peng Yuan, Zhang Lei, Lawrie Charles H
Sino-Swiss Institute of Advanced Technology (SSIAT), Shanghai University, Shanghai 201899, China.
School of Microelectronics, Shanghai University, Shanghai 201899, China.
ACS Omega. 2024 Aug 19;9(35):37163-37169. doi: 10.1021/acsomega.4c04385. eCollection 2024 Sep 3.
The biosensing field faces a significant challenge in efficiently detecting multiple analytes in a single diagnostic sample in order to compete with other established multiplex molecular diagnostic technologies such as PCR and ELISA. In response, we have developed a colorimetric nanobiosensor based on multiple morphological forms of functionalized gold nanoparticles (AuNPs) for the simultaneous detection of the influenza virus and SARS-CoV-2 virus. Gold nanospheres (GNSp) were modified with oligonucleotides specific for the influenza A virus, while gold nanoshells (GNSh) were modified with oligonucleotides specific for the SARS-CoV-2 virus. In the presence of their respective targets, AuNPs remain stable due to DNA-DNA interactions; conversely, in the absence of targets, AuNPs aggregate. Consequently, the hybrid system exhibits an indigo color with the SARS-CoV-2 target, a blue color with the Influenza A target, and a purple color with both targets, visible to the naked eye. Analytical sensitivity was 100 nM, and no cross-reactivity was observed with potentially confounding pathogens. This approach holds great promise for the simultaneous identification of multiple pathogens in a rapid manner without the need for equipment or trained personnel.
为了与其他成熟的多重分子诊断技术（如聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA））竞争，生物传感领域在有效检测单个诊断样本中的多种分析物方面面临重大挑战。作为回应，我们开发了一种基于功能化金纳米颗粒（AuNP）多种形态的比色纳米生物传感器，用于同时检测流感病毒和严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒。用针对甲型流感病毒的寡核苷酸修饰金纳米球（GNSp），而用针对SARS-CoV-2病毒的寡核苷酸修饰金纳米壳（GNSh）。在各自靶标的存在下，由于DNA-DNA相互作用，AuNP保持稳定；相反，在没有靶标的情况下，AuNP聚集。因此，该混合系统在存在SARS-CoV-2靶标时呈现靛蓝色，在存在甲型流感靶标时呈现蓝色，在同时存在两种靶标时呈现紫色，肉眼可见。分析灵敏度为100 nM，未观察到与潜在混淆病原体的交叉反应。这种方法在无需设备或专业人员的情况下，快速同时鉴定多种病原体方面具有巨大潜力。
