绿藻分离株对MCF-7乳腺癌细胞的抗菌及细胞毒性作用

Antimicrobial and Cytotoxic Effect of Green Algae Isolate on MCF-7 Breast Cancer.

作者信息

Hadisaputri Yuni Elsa, Soekamto Nunuk Hariani, Pudjiastuti Pratiwi, Aristokrat Aria, Bahrun Bahrun, Abdullah Fajar Fauzi, Okino Tatsufumi

机构信息

Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, 45363, Indonesia.

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia.

出版信息

Breast Cancer (Dove Med Press). 2024 Sep 4;16:555-565. doi: 10.2147/BCTT.S469036. eCollection 2024.

INTRODUCTION

Marine algae are increasingly becoming a potential resource for new drugs. In recent decades, including (). Meanwhile, antimicrobial and anticancer agents are the first line of choice for developing alternative compounds, considering the annually increasing resistant events. Therefore, this study aimed to examine the antimicrobial and cytotoxic potential of isolate compounds.

METHODS

The resulted in 2 compounds, sitosterol 3β tetracosanoate and (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol. Both compounds were tested to have antibacterial effects against , , , (MRSA). Proliferation assay was conducted using the PrestoBlue™ Cell Viability Reagent, which was also used to measure the IC against MCF-7 breast cancer cells.

RESULTS

The results showed that (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol had antimicrobial activity against and IC value of 142.18 µg/mL against MCF-7 cells, while sitosterol 3β tetracosanoate does not have any antimicrobial activity and IC value of 681.65 µg/mL. Moreover, the mechanism prediction using docking with caspase-3 receptor to induce apoptosis was also evaluated.

CONCLUSION

Based on the results, (E)-17-(8-ethyl-4,5,9-trimethyldec-6-en-2-yl)-13-methyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol of has great potential as an antimicrobial and anticancer agent.

引言

海藻正日益成为新药的潜在资源。在最近几十年里，包括（）。同时，考虑到耐药事件逐年增加，抗菌和抗癌药物是开发替代化合物的首选。因此，本研究旨在检测分离化合物的抗菌和细胞毒性潜力。

方法

（此处英文不完整，无法准确翻译）得到了2种化合物，即3β-二十四烷酸甾醇酯和（E）-17-（8-乙基-4,5,9-三甲基癸-6-烯-2-基）-13-甲基-2,3,4,7,8,9,10,11,12,13,14,15,16,17-十四氢-1H-环戊并[a]菲-3-醇。对这两种化合物进行了测试，以检测其对（此处英文不完整，无法准确翻译）、（此处英文不完整，无法准确翻译）、（此处英文不完整，无法准确翻译）、耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌效果。使用PrestoBlue™细胞活力试剂进行增殖测定，该试剂也用于测量对MCF-7乳腺癌细胞的半数抑制浓度（IC）。

结果

结果表明，（E）-17-（8-乙基-4,5,9-三甲基癸-6-烯-2-基）-13-甲基-2,3,4,7,8,9,10,11,12,13,14,15,16,17-十四氢-1H-环戊并[a]菲-3-醇对（此处英文不完整，无法准确翻译）具有抗菌活性，对MCF-7细胞的IC值为142.18µg/mL，而3β-二十四烷酸甾醇酯没有任何抗菌活性，IC值为681.65µg/mL。此外，还评估了通过与半胱天冬酶-3受体对接来诱导细胞凋亡的机制预测。