兔乳腺一种主要的N-糖基化蛋白的鉴定及其在体内发育过程中的出现情况。
Identification of a major N-glycosylated protein of rabbit mammary gland and its appearance during development in vivo.
作者信息
Bradshaw J P, White D A
出版信息
Int J Biochem. 1985;17(2):175-85. doi: 10.1016/0020-711x(85)90112-0.
PMID:3924677
Abstract
A major N-glycosylated protein was purified from hormonally stimulated pregnant and lactating rabbit mammary tissue. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, immunodiffusion and amino acid analysis showed the protein to be transferrin. The rate of synthesis of the protein during tissue development was studied and found to parallel the whey proteins casein and alpha-lactalbumin. The function of the protein during lactation is discussed.
摘要
从经激素刺激的怀孕和哺乳期兔乳腺组织中纯化出一种主要的N-糖基化蛋白。在十二烷基硫酸钠存在下进行的聚丙烯酰胺凝胶电泳、免疫扩散和氨基酸分析表明该蛋白为转铁蛋白。研究了该蛋白在组织发育过程中的合成速率，发现其与乳清蛋白酪蛋白和α-乳白蛋白平行。讨论了该蛋白在泌乳过程中的功能。
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