Yu Wenxin, Kastriti Maria Eleni, Ishan Mohamed, Choudhary Saurav Kumar, Rashid Md Mamunur, Kramer Naomi, Do Hy Gia Truong, Wang Zhonghou, Xu Ting, Schwabe Robert F, Ye Kaixiong, Adameyko Igor, Liu Hong-Xiang
Department of Animal and Dairy Science, Regenerative Bioscience Center, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA, United States.
Department of Neuroimmunology, Medical University of Vienna, Vienna, Austria.
Front Cell Dev Biol. 2024 Aug 23;12:1460669. doi: 10.3389/fcell.2024.1460669. eCollection 2024.
We have recently demonstrated that -expressing ( ) cells give rise to mainly type-III neuronal taste bud cells that are responsible for sour and salt taste. The two tissue compartments containing cells in the surrounding of taste buds include the connective tissue core of taste papillae and von Ebner's glands (vEGs) that are connected to the trench of circumvallate and foliate papillae.
In this study, we performed single cell RNA-sequencing of the epithelium of mouse circumvallate/vEG complex and used inducible Cre mouse models to map the cell lineages of vEGs and/or connective tissue (including stromal and Schwann cells).
Transcriptomic analysis indicated that expression was enriched in the cell clusters of vEG ducts that contained abundant proliferating cells, while expression was enriched in type-III taste bud cells and vEG ductal cells. lineage mapping showed that the traced cells were distributed in circumvallate taste buds concurrently with those in the vEGs, but not in the connective tissue. Moreover, multiple genes encoding pathogen receptors were enriched in the vEG ducts hosting cells.
Our data supports that it is the vEGs, not connective tissue core, that serve as the niche of taste bud progenitors. If this is also true in humans, our data indicates that vEG duct is a source of taste bud progenitors and susceptible to pathogen infections.
我们最近证明，表达 - 的（ ）细胞主要产生负责酸味和咸味的III型神经元味蕾细胞。味蕾周围含有 细胞的两个组织隔室包括味乳头的结缔组织核心和与轮廓乳头和叶状乳头沟相连的埃伯纳腺（vEGs）。
在本研究中，我们对小鼠轮廓乳头/vEG复合体的上皮进行了单细胞RNA测序，并使用诱导型Cre小鼠模型来绘制vEGs和/或结缔组织（包括基质细胞和雪旺细胞）的细胞谱系。
转录组分析表明， 在含有丰富增殖细胞的vEG导管细胞簇中表达富集，而 在III型味蕾细胞和vEG导管细胞中表达富集。 谱系映射显示，追踪到的细胞与vEGs中的细胞同时分布在轮廓乳头味蕾中，但不在结缔组织中。此外，在含有 细胞的vEG导管中，多个编码病原体受体的基因富集。
我们的数据支持，作为 味蕾祖细胞生态位的是vEGs，而不是结缔组织核心。如果在人类中也是如此，我们的数据表明vEG导管是 味蕾祖细胞的来源，并且易受病原体感染。
