Toh H, Rittman G, Mackenzie I C
Fukuoka Dental College, Japan.
Epithelial Cell Biol. 1993 Jul;2(3):126-33.
The patterns of keratin expression of taste buds in murine oral mucosa were examined using a panel of antibodies with various specificities for cytokeratins. The patterns for taste buds differed markedly from those of the surrounding epithelium but no regional differences in the staining patterns of the taste buds themselves were detected. The taste buds and the ducts of von Ebner's glands were strongly stained by monoclonal antibodies (mAbs) against cytokeratins (K) 8, 18 and 19, those typically expressed by simple epithelia. Merkel cells, which are also present in the oral epithelium and may correspond to the type III cells in taste buds, stained for K8 and K18 but not K19. None of the mAbs against simple epithelial keratins stained the stratifying epithelium of the trench wall or of the oral mucosa. Antibodies with specificity for keratins that are expressed as differentiation products of the mucosal epithelium did not stain taste buds. The staining pattern of the epithelium of the trench wall indicated that it expressed some keratins typical of stratifying epithelia but lacked the full pattern of differentiation of the adjacent mucosal epithelia. Staining within the taste buds was not homogeneous but no clear differences of keratin staining could be directly related to the subtypes of cells constituting them. The markedly differing patterns of keratin expression between taste buds and the adjacent epithelium raises questions about the type of inductive signals that produce and maintain these patterns.
利用一组对细胞角蛋白具有不同特异性的抗体，研究了小鼠口腔黏膜味蕾的角蛋白表达模式。味蕾的模式与周围上皮细胞的模式明显不同，但未检测到味蕾自身染色模式的区域差异。味蕾和味腺导管被抗细胞角蛋白（K）8、18和19的单克隆抗体（mAb）强烈染色，这些是典型的由单层上皮表达的角蛋白。默克尔细胞也存在于口腔上皮中，可能与味蕾中的III型细胞相对应，其对K8和K18染色，但对K19不染色。针对单层上皮角蛋白的单克隆抗体均未对沟壁或口腔黏膜的复层上皮染色。对作为黏膜上皮分化产物表达的角蛋白具有特异性的抗体未对味蕾染色。沟壁上皮的染色模式表明，它表达了一些复层上皮典型的角蛋白，但缺乏相邻黏膜上皮的完整分化模式。味蕾内的染色不均匀，但角蛋白染色的明显差异不能直接与构成它们的细胞亚型相关。味蕾与相邻上皮细胞之间角蛋白表达模式的显著差异，引发了关于产生和维持这些模式的诱导信号类型的问题。
