University of Lodz, Faculty of Biology and Environmental Protection, Department of Oncobiology and Epigenetics, Pomorska 141/143, 90-236 Lodz, Poland.
University of Lodz, Faculty of Biology and Environmental Protection, Department of Oncobiology and Epigenetics, Pomorska 141/143, 90-236 Lodz, Poland,
Cell Physiol Biochem. 2024 Sep 7;58(5):459-476. doi: 10.33594/000000725.
BACKGROUND/AIMS: One of the treatments for breast cancer is surgical resection of the tumour and prevention of recurrence with postoperative radiotherapy. Unfortunately, radiotherapy is not always effective enough due to the low sensitivity of cancer cells to ionising radiation. This study aimed to evaluate the radiosensitising properties of resveratrol, piceatannol and polydatin on breast cancer cells, which differ in the presence of hormonal receptors on their surface.
The experimental part was carried out on triple-negative breast cancer cells (HCC38) and hormone-dependent cells (MCF7). The study assessed the level of cell death, changes in the expression of genes (BAX, BCL-2) and proteins related to the apoptosis process (CASPASE 3, 8 and P53), changes in the expression of antioxidant enzymes (CATALASE, SOD, GPx1/2) and NRF-2. Additionally, the expression level of RAD51 protein and histone H2AX, which are involved in DNA repair processes, was assessed. Statistical significance was evaluated by a two-way analysis of variance (ANOVA) followed by Tukey's post hoc test (p < 0.05).
Ionising radiation in combination with resveratrol or piceatannol activates the apoptosis process by internal and external pathways. Greater sensitivity of MCF7 cells compared to HCC38 cells to ionising radiation in combination with resveratrol is associated with a weaker antioxidant response of cells and reduced intensity of DNA damage repair. DNA repair induced by ionising radiation occurs more effectively in HCC38 cells than in MCF7 cells.
Resveratrol has the highest radiosensitising potential among the tested stilbene for cells of both lines. The effectiveness of ionizing radiation in combination with resveratrol (to a lesser extent with piceatannol) is more significant in MCF7 than in HCC38 cells.
背景/目的：乳腺癌的一种治疗方法是手术切除肿瘤，并在术后进行放疗以预防复发。不幸的是，由于癌细胞对电离辐射的敏感性较低，放疗并不总是足够有效。本研究旨在评估白藜芦醇、白皮杉醇和虎杖苷对乳腺癌细胞的放射增敏特性，这些细胞在表面是否存在激素受体上有所不同。
实验部分在三阴性乳腺癌细胞（HCC38）和激素依赖性细胞（MCF7）上进行。该研究评估了细胞死亡水平、基因（BAX、BCL-2）和与细胞凋亡过程相关的蛋白质（CASPASE 3、8 和 P53）表达的变化、抗氧化酶（CATALASE、SOD、GPx1/2）和 NRF-2 的表达变化，以及参与 DNA 修复过程的 RAD51 蛋白和组蛋白 H2AX 的表达水平。统计显著性通过双向方差分析（ANOVA）进行评估，然后进行 Tukey 事后检验（p < 0.05）。
电离辐射与白藜芦醇或白皮杉醇联合使用可通过内、外途径激活细胞凋亡过程。与 HCC38 细胞相比，MCF7 细胞对电离辐射与白藜芦醇联合使用的敏感性更强，这与细胞抗氧化反应较弱和 DNA 损伤修复强度降低有关。与 MCF7 细胞相比，HCC38 细胞中的电离辐射诱导的 DNA 修复更为有效。
在测试的三种二苯乙烯中，白藜芦醇对两种细胞系的细胞具有最高的放射增敏潜力。电离辐射与白藜芦醇（在较小程度上与白皮杉醇）联合使用的效果在 MCF7 细胞中比在 HCC38 细胞中更为显著。
