Magnusson C G, Masson P L
Int Arch Allergy Appl Immunol. 1985;77(3):292-9. doi: 10.1159/000233834.
We have assayed IgE in various human secretions by particle-counting immunoassay (PACIA). The introduction of a pepsin digestion step of the sample prior to the assay not only eliminates possible interfering factors but also releases from IgE the protease-resistant Fc"epsilon fragment. Since this fragment could represent the residual form of IgE in certain secretions rich in proteases it should be a suitable calibrator for the assay of IgE in secretions. The concentrations of IgE that we found in most fluids (urine, saliva, tears, milk, aqueous humour, cerebrospinal fluid, broncho-alveolar washings, intestinal fluids and faecal extracts) were up to 100 times lower than those reported by others who have used the radioimmunosorbent test (RIST). We suspect that the high values found with RIST are related to the susceptibility of this test to various interfering factors and not to a local production of IgE in most secretions of normal individuals.
我们通过粒子计数免疫测定法（PACIA）检测了各种人体分泌物中的IgE。在检测前对样本进行胃蛋白酶消化步骤，不仅消除了可能的干扰因素，还从IgE中释放出抗蛋白酶的Fcε片段。由于该片段可能代表富含蛋白酶的某些分泌物中IgE的残留形式，因此它应该是分泌物中IgE检测的合适校准物。我们在大多数液体（尿液、唾液、眼泪、乳汁、房水、脑脊液、支气管肺泡灌洗液、肠液和粪便提取物）中发现的IgE浓度比使用放射免疫吸附试验（RIST）的其他人报告的浓度低多达100倍。我们怀疑RIST检测到的高值与该检测对各种干扰因素的敏感性有关，而不是与正常个体大多数分泌物中IgE的局部产生有关。
