Department of Histology and Embryology, Youjiang Medical University for Nationalities.
Department of Geriatric Orthopedics, Affiliated Hospital of Youjiang Medical University for Nationalities.
J Vis Exp. 2024 Aug 23(210). doi: 10.3791/67051.
Osteoporosis is a common metabolic disease of elderly and postmenopausal women, with no obvious symptoms during its early stages. In the latter stages of this condition, the patients are prone to fractures, and this can seriously affect their health and quality of life. The worldwide increase in life expectancy has made osteoporosis a global concern. The Xiaoyao pills were previously used in the treatment of depression. In addition, the drug appeared to have estrogen-like activity, which affected the expression of ALP, an early osteoblast-specific marker, and COL-1, a major component of bone extracellular matrix. Xiaoyao pills were assessed for their effects on postmenopausal osteoporosis (PMOM) in mice. The target information of each herbal component of Xiaoyao pills was accessed through the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. Information from GeneCards, OMIM, PharmGkb, TTD, DrugBank, and other websites was used to construct the regulatory network of the herbal complex through Cytoscape and String network to assess the protein interactions. Mice were ovariectomized, and treated with high and low doses of Xiaoyao pills and these were compared to controls. Their symptoms were assessed by immunocytochemistry of bone tissues. The results suggested that Xiaoyao pills had the ability to alleviate the symptoms of PMOM in ovariectomized mice through the IL-17 signaling pathway. This drug has the potential to become a novel therapeutic agent for the treatment of osteoporosis.
骨质疏松症是一种常见的老年和绝经后妇女代谢性疾病，早期阶段无明显症状。在该疾病的晚期，患者容易骨折，这会严重影响他们的健康和生活质量。全球预期寿命的延长使骨质疏松症成为全球关注的问题。逍遥丸以前用于治疗抑郁症。此外，该药物似乎具有雌激素样活性，影响了成骨细胞早期特异性标志物 ALP 和骨细胞外基质主要成分 COL-1 的表达。研究人员评估了逍遥丸对去卵巢骨质疏松症（PMOM）的作用。通过中药系统药理学（TCMSP）数据库获取逍遥丸中每个草药成分的靶标信息。通过 Cytoscape 和 String 网络从 GeneCards、OMIM、PharmGkb、TTD、DrugBank 等网站获取信息，构建草药复合物的调控网络，评估蛋白质相互作用。通过对骨组织的免疫细胞化学评估，对去卵巢的小鼠进行逍遥丸高、低剂量治疗，并与对照组进行比较，以评估其症状。结果表明，逍遥丸通过 IL-17 信号通路缓解去卵巢小鼠 PMOM 的症状。该药物有可能成为骨质疏松症治疗的一种新型治疗剂。
