Xin Xin, Wang Shu, Pan Yunjiao, Ye Linhan, Zhai Tingting, Gu Mengjie, Wang Yanjiao, Zhang Jiedao, Li Xiang, Yang Wei, Zhang Shuxin
National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, China.
Plant Cell Environ. 2025 Jan;48(1):97-108. doi: 10.1111/pce.15144. Epub 2024 Sep 9.
The freezing temperature greatly limits the growth, development and productivity of plants. The C-repeat/DRE binding factor (CBF) plays a major role in cold acclimation, enabling plants to increase their freezing tolerance. Notably, the INDUCER OF CBF EXPRESSION1 (ICE1) protein has garnered attention for its pivotal role in bolstering plants' resilience against freezing through transcriptional upregulation of DREB1A/CBF3. However, the research on the interaction between ICE1 and other transcription factors and its function in regulating cold stress tolerance is largely inadequate. In this study, we found that a R2R3 MYB transcription factor CDC5 interacts with ICE1 and regulates the expression of CBF3 by recruiting RNA polymerase II, overexpression of ICE1 can complements the freezing deficient phenotype of cdc5 mutant. CDC5 increases the expression of CBF3 in response to freezing. Furthermore, CDC5 influences the expression of CBF3 by altering the chromatin status through H3K4me3 and H3K27me3 modifications. Our work identified a novel component that regulates CBF3 transcription in both ICE1-dependent and ICE1-independent manner, improving the understanding of the freezing signal transduction in plants.
冰点温度极大地限制了植物的生长、发育和生产力。C重复序列/脱水应答元件结合因子（CBF）在植物冷驯化过程中起主要作用，使植物能够提高其抗冻性。值得注意的是，CBF表达诱导因子1（ICE1）蛋白因其通过转录上调DREB1A/CBF3在增强植物抗冻能力方面的关键作用而受到关注。然而，关于ICE1与其他转录因子之间的相互作用及其在调节冷胁迫耐受性中的功能的研究在很大程度上还不够充分。在本研究中，我们发现一个R2R3 MYB转录因子CDC5与ICE1相互作用，并通过招募RNA聚合酶II来调节CBF3的表达，过表达ICE1可以弥补cdc5突变体的抗冻缺陷表型。CDC5响应冷冻增加CBF3的表达。此外，CDC5通过H3K4me3和H3K27me3修饰改变染色质状态来影响CBF3的表达。我们的工作鉴定出了一个以依赖ICE1和不依赖ICE1的方式调节CBF3转录的新组分，增进了对植物冷冻信号转导的理解。
