适合作为免疫抗原的猴和人乳酸脱氢酶同工酶5的生化纯化
Biochemical purification of monkey and human LDH isoenzyme 5 suitable as immunization antigen.
作者信息
Paoli C, Bardelli F, Rinaldi F, Donnini F, Tarli P
出版信息
Ital J Biochem. 1985 Jan-Feb;34(1):11-8.
PMID:3924858
Abstract
A method consisting in an ion-exchange chromatography on DEAE-Sephadex A50 and an affinity chromatography on Oxamate-hexyl-Sepharose for the purification of monkey and human LDH isoenzyme 5 is presented. Analytical isoelectric focusing reveals minor variants in the monkey LDH isoenzyme 5. This material has been used to produce an antiserum from rabbits which recognizes all the human LDH isoenzymes containing the M subunit, without cross-reacting with LDH1.
摘要
本文介绍了一种通过在DEAE-葡聚糖A50上进行离子交换色谱以及在草氨酸己基-琼脂糖上进行亲和色谱来纯化猴和人乳酸脱氢酶同工酶5的方法。分析性等电聚焦显示猴乳酸脱氢酶同工酶5存在少量变体。该材料已用于从兔制备抗血清，该抗血清可识别所有含M亚基的人乳酸脱氢酶同工酶，而不与乳酸脱氢酶1发生交叉反应。
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