Bioproduction Research Institute, Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan.
Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, 903-0213, Japan.
Microb Ecol. 2024 Sep 9;87(1):112. doi: 10.1007/s00248-024-02426-8.
A number of insects are associated with gut symbiotic microorganisms, wherein symbiotic partners play pivotal metabolic roles for each other such as nutrient supplementation, diet degradation, and pesticide detoxification. Despite the ecological and evolutionary importance of gut microbial communities in insects, their diversity and dynamics remain unclear in many species. The green plant bug Apolygus spinolae, a notorious grapevine pest in Japan, damages grape shoots and severely reduces grape berry yield and quality. The plant bug possesses a simple tubular gut housing ~ 10 bacteria. Here, we investigated geographic, seasonal, and growth-related dynamics of gut microbiota by high-throughput sequencing in 82 individuals (11 nymphs and 71 adults) from five locations in Hokkaido, Japan. In plant bugs, gut microbiota changed dynamically depending on region, season, and developmental stage. Among the gut bacteria, Serratia was consistently and abundantly detected and was significantly affected by seasonal changes. In addition, Caballeronia, known as a specific symbiont in some stinkbug species, was abundantly detected, especially in insects collected in late summer despite A. spinolae complete lack of midgut crypts known as symbiotic organ harboring Caballeronia in other stinkbug species. Considering their prevalence among host bug populations, it is possible these gut microorganisms play a pivotal role in the adaptation of the green plant bug to grapevine fields, although further confirmation through rearing experiments is needed.
许多昆虫与肠道共生微生物有关，其中共生伙伴在彼此的代谢中起着关键作用，例如营养补充、饮食降解和农药解毒。尽管肠道微生物群落对昆虫具有生态和进化重要性，但它们的多样性和动态在许多物种中仍不清楚。绿盲蝽 Apolygus spinolae 是日本一种臭名昭著的葡萄藤害虫，它会损害葡萄嫩枝，严重降低葡萄浆果的产量和质量。这种植食性盲蝽拥有一个简单的管状肠道，容纳着大约 10 种细菌。在这里，我们通过在日本北海道的五个地点采集的 82 个个体（11 个若虫和 71 个成虫）的高通量测序，研究了肠道微生物群的地理、季节性和与生长相关的动态。在植食性盲蝽中，肠道微生物群根据地区、季节和发育阶段而动态变化。在肠道细菌中， Serratia 一直被大量检测到，并受到季节性变化的显著影响。此外，Caballeronia 作为一些臭虫物种的特定共生体被大量检测到，特别是在夏季末采集的昆虫中，尽管 A. spinolae 完全缺乏中肠隐窝，这些隐窝是其他臭虫物种中 Caballeronia 共生体的栖息器官。考虑到它们在宿主昆虫种群中的普遍性，这些肠道微生物可能在绿盲蝽适应葡萄藤领域中起着关键作用，尽管需要通过饲养实验进一步确认。
