白杨素可下调泡沫细胞中 ADAMTS-4 的表达。
Chrysin downregulates the expression of ADAMTS-4 in foam cells.
机构信息
Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, 695581, India.
Department of Biotechnology, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, 695581, India.
出版信息
Mol Biol Rep. 2024 Sep 9;51(1):968. doi: 10.1007/s11033-024-09896-6.
BACKGROUND
Chrysin, a polyphenolic compound, possesses antioxidant and anti-inflammatory properties. In this study, we investigated the effect of chrysin on the expression of A disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), a protease enzyme involved in degrading extracellular matrix associated with atherosclerosis.
METHODS AND RESULTS
We have studied the cell viability by MTT assay and foam cell formation by oil red O staining. The mRNA and protein expression of ADAMTS-4 was studied using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Our study showed that chrysin significantly downregulates the expression of ADAMTS-4 in foam cells.
CONCLUSION
Chrysin's ability to downregulate the expression of ADAMTS-4, a protease involved in degrading the extracellular matrix, bestows upon it a new therapeutic potential for managing atherosclerosis.
背景
白杨素是一种多酚化合物，具有抗氧化和抗炎特性。在这项研究中，我们研究了白杨素对参与降解与动脉粥样硬化相关的细胞外基质的蛋白酶 ADAMTS-4（解整合素和金属蛋白酶与凝血酶样 4）表达的影响。
方法和结果
我们通过 MTT assay 检测细胞活力，通过油红 O 染色检测泡沫细胞形成。使用定量聚合酶链反应 (qPCR) 和 Western blot 分别研究 ADAMTS-4 的 mRNA 和蛋白表达。我们的研究表明，白杨素可显著下调泡沫细胞中 ADAMTS-4 的表达。
结论
白杨素下调参与降解细胞外基质的蛋白酶 ADAMTS-4 的表达的能力为其治疗动脉粥样硬化提供了新的治疗潜力。
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