Dima Francesco, Salvagno Gian Luca, Lippi Giuseppe
Section of Clinical Biochemistry and School of Medicine, University of Verona, Verona, Italy.
Section of Clinical Biochemistry and School of Medicine, University of Verona, Verona, Italy.
Biomed J. 2024 Dec;47(6):100787. doi: 10.1016/j.bj.2024.100787. Epub 2024 Sep 7.
We planned a series of experiments to investigate the possible role of spike protein of different SARS-CoV-2 variants in influencing erythrocyte biology. The values of erythrocyte count, hemoglobin, and mean corpuscular hemoglobin (MHC) did not vary across all samples challenged with both concentrations of the four different SARS-CoV-2 recombinant spike proteins. A significant increase in mean corpuscular volume (MCV) was observed with the recombinant SARS-CoV-2 Alpha and Delta spike proteins at both 2 and 20 ng/mL final concentrations. Red blood cell distribution width (RDW) values increased significantly in samples treated with 20 ng/mL of all SARS-CoV-2 recombinant spike proteins and reached the highest values in samples treated with Omicron recombinant spike protein. Blood smear revision evidenced hemagglutination and rouleaux in samples to which recombinant SARS-CoV-2 spike proteins were added, especially in those with Alpha and Delta variants.
我们计划开展一系列实验,以研究不同新冠病毒变异株的刺突蛋白在影响红细胞生物学方面可能发挥的作用。在用四种不同的新冠病毒重组刺突蛋白的两种浓度进行攻击的所有样本中,红细胞计数、血红蛋白和平均红细胞血红蛋白含量(MCH)的值均无变化。在终浓度为2 ng/mL和20 ng/mL的重组新冠病毒阿尔法和德尔塔刺突蛋白处理组中,平均红细胞体积(MCV)显著增加。在用20 ng/mL的所有新冠病毒重组刺突蛋白处理的样本中,红细胞分布宽度(RDW)值显著增加,在用奥密克戎重组刺突蛋白处理的样本中达到最高值。血涂片检查证明,在添加了重组新冠病毒刺突蛋白的样本中出现了血细胞凝集和缗钱状形成,尤其是在含有阿尔法和德尔塔变异株的样本中。