Yang Jing, Xin Ruodong, Lehmkuhl Sören, Korvink Jan G, Brandner Jürgen J
Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), 76344, Eggenstein-Leopoldshafen, Germany.
Karlsruhe Nano Micro Facility (KNMFi), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Sci Rep. 2024 Sep 9;14(1):21022. doi: 10.1038/s41598-024-71354-x.
SABRE is emerging as a fast, simple and low-cost hyperpolarization method because of its ability to regenerate enhanced NMR signals. Generally, SABRE hyperpolarization has been performed predominantly manually, leading to variations in reproducibility and efficiency. Recent advances in SABRE include the development of automated shuttling systems to address previous inconsistencies. However, the operational complexity of such systems and the challenges of integration with existing workflows hinder their widespread adoption. This work presents a fully automated lab workstation based on a benchtop NMR spectrometer, specifically designed to facilitate SABRE of different nuclei across different polarization fields. We demonstrated the capability of this system through a series of routine SABRE experimental protocols, including consecutive SABRE hyperpolarization with high reproducibility (average standard deviation of 1.03%), optimization polarization of C nuclei respect to the polarization transfer field, and measurement of polarization buildup rate or decay time across a wide range of magnetic fields. Furthermore, we have iteratively optimized the durations for pulsed SABRE-SHEATH C pyruvate. The constructed SABRE workstation offers full automation, high reproducibility, and functional diversification, making it a practical tool for conducting routine SABRE hyperpolarization experiments. It provides a robust platform for high-throughput and reliable SABRE and X-SABRE hyperpolarization studies.
由于能够再生增强的核磁共振信号，SABRE正成为一种快速、简单且低成本的超极化方法。一般来说，SABRE超极化主要是手动进行的，导致重现性和效率存在差异。SABRE的最新进展包括开发自动穿梭系统以解决先前的不一致问题。然而，此类系统的操作复杂性以及与现有工作流程集成的挑战阻碍了它们的广泛采用。这项工作展示了一种基于台式核磁共振光谱仪的全自动实验室工作站，专门设计用于促进不同原子核在不同极化场中的SABRE操作。我们通过一系列常规SABRE实验方案展示了该系统的能力，包括具有高重现性（平均标准偏差为1.03%）的连续SABRE超极化、相对于极化转移场优化C原子核的极化，以及在广泛磁场范围内测量极化建立速率或衰减时间。此外，我们还迭代优化了脉冲SABRE-SHEATH C丙酮酸的持续时间。构建的SABRE工作站提供了全自动化、高重现性和功能多样化，使其成为进行常规SABRE超极化实验的实用工具。它为高通量且可靠的SABRE和X-SABRE超极化研究提供了一个强大的平台。
