Tochikubo K, Yasuda Y
Microbiol Immunol. 1985;29(3):213-28. doi: 10.1111/j.1348-0421.1985.tb00821.x.
Both a salt-dependent form and an active form of glucose dehydrogenase [EC 1.1.1.47] were isolated from germinated spores of Bacillus subtilis disrupted in deionized water and 100 mM phosphate buffer (pH 6.6), and most of the enzyme isolated from young vegetative cells was the active form regardless of the conditions for breakage by sonication. The molecular weight of the salt-dependent form of the enzyme was about 55,000 and that of the active form was about 120,000. From the above results and the results on the glucose dehydrogenase extracted from resting spores disrupted in deionized water and 100 mM phosphate buffer (pH 6.6) reported in a previous paper, we propose that glucose dehydrogenase is present in resting spores as a monomeric form and is activated with association in vivo during germination and outgrowth.
从在去离子水和100 mM磷酸盐缓冲液（pH 6.6）中破碎的枯草芽孢杆菌萌发孢子中分离出了盐依赖性形式和活性形式的葡萄糖脱氢酶[EC 1.1.1.47]，并且无论通过超声破碎的条件如何，从年轻营养细胞中分离出的大部分酶都是活性形式。该酶盐依赖性形式的分子量约为55,000，活性形式的分子量约为120,000。根据上述结果以及先前论文中报道的从在去离子水和100 mM磷酸盐缓冲液（pH 6.6）中破碎的静止孢子中提取的葡萄糖脱氢酶的结果，我们提出葡萄糖脱氢酶在静止孢子中以单体形式存在，并在萌发和生长过程中在体内通过缔合而被激活。
