Yoshioka Y, Ose Y, Sato T
Sci Total Environ. 1985 May;43(1-2):149-57. doi: 10.1016/0048-9697(85)90037-3.
A test was developed using Tetrahymena pyriformis in order to determine the toxicity of various chemicals. Pre-cultured T. pyriformis was exposed for 24 h at 30 degrees C to various concentrations of chemicals, and the number of T. pyriformis surviving were then counted. The concentration of the chemical, at which the proliferation of T. pyriformis was restricted to one-half of the blank test (EC50), was determined. The method, applied to 57 chemicals, demonstrated that it could be used to detect the chemicals at low concentrations rapidly and with ease. The EC50 values showed a good relationship with 48 h LC50 values for Himedaka (Orizias latipes), and could be explained on the basis of the partition coefficient between water and n-octanol.
为了确定各种化学物质的毒性，开发了一种使用梨形四膜虫的测试方法。将预培养的梨形四膜虫在30℃下暴露于各种浓度的化学物质中24小时，然后计算存活的梨形四膜虫数量。确定了梨形四膜虫增殖受限至空白试验一半时的化学物质浓度（EC50）。该方法应用于57种化学物质，结果表明它可用于快速、轻松地检测低浓度的化学物质。EC50值与青鳉（Orizias latipes）的48小时LC50值显示出良好的关系，并且可以根据水和正辛醇之间的分配系数来解释。
