Anhui Provincial Key Laboratory of Hazardous Factors and Risk Control of Agri-food Quality Safety, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China.
Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
J Agric Food Chem. 2024 Sep 25;72(38):21112-21121. doi: 10.1021/acs.jafc.4c03737. Epub 2024 Sep 10.
Acetochlor residues can contaminate anoxic habitats where anaerobic microbial transformation dominates. Herein, a highly efficient anaerobic acetochlor-degrading consortium ACT6 was enriched using sulfate and acetochlor as selection pressures. The acclimated consortium ACT6 showed an 8.7-fold increase in its ability to degrade acetochlor compared with the initial consortium ACT1. Two degradation pathways of acetochlor were found: reductive dechlorination and thiol-substitution dechlorination in the chloroacetyl group, in which the latter dominated. Acclimation enhanced the abundances of , , and from 0.7 to 28.0% (40-fold), 4.7 to 18.1% (4-fold), and 2.3 to 12.3% (5-fold), respectively, which were positively correlated with sulfate concentrations and acetochlor degradation ability. Three acetochlor-degrading anaerobes were isolated from the acclimated consortium ACT6, namely sp. SRB-5, sp. BAD-10, and sp. BAD-7. This study provides new insights into the anaerobic catabolism of acetochlor and the anaerobic treatment of acetochlor in wastewater.
乙酰甲草胺残留会污染缺氧环境，而在缺氧环境中，厌氧微生物转化占主导地位。本文以硫酸盐和乙酰甲草胺为选择压力，富集得到了一种高效的厌氧乙酰甲草胺降解菌群落 ACT6。与初始菌群落 ACT1 相比，驯化后的菌群落 ACT6 降解乙酰甲草胺的能力提高了 8.7 倍。发现了两种降解乙酰甲草胺的途径：氯乙酰基的还原脱氯和巯基取代脱氯，其中后者占主导地位。驯化提高了、和的丰度，分别从 0.7%增加到 28.0%（40 倍）、4.7%增加到 18.1%（4 倍）和 2.3%增加到 12.3%（5 倍），这与硫酸盐浓度和乙酰甲草胺降解能力呈正相关。从驯化后的菌群落 ACT6 中分离出三种能够降解乙酰甲草胺的厌氧微生物，分别为 sp. SRB-5、 sp. BAD-10 和 sp. BAD-7。本研究为乙酰甲草胺的厌氧代谢和废水的厌氧处理提供了新的见解。
