Gallicchio V S, Chen M G, Watts T D
Acta Haematol. 1985;73(2):80-5. doi: 10.1159/000206285.
The ability of the anabolic steroid nandrolone decanoate (ND) to increase megakaryocytopoiesis (MP) and the hematopoietic-inductive microenvironment (HIM) after sublethal irradiation was evaluated. Immediately after receiving whole body irradiation (2 Gy) and on the next 2 days, mice were injected with 1.25 mg of ND in propylene glycol intraperitoneally. Irradiated control mice received only vehicle. Compared to controls, drug-treated mice showed a 151% increase in the platelet level on day 14. Drug-treated mice also demonstrated a significant rebound in MP, as colony-forming progenitor stem cells (CFU-Mk) from bone marrow rose to 288% of control by day 8 and from spleen to 599% by day 1 following the last drug injection. In addition, drug-treated mice produced a 286% increase in bone marrow and a 378% in splenic stromal cell colonies on day 5 following drug treatment. These results demonstrate that ND enhances MP and HIM following sublethal irradiation. After hematopoietic suppression, ND may serve to stimulate MP and HIM recovery both at the stem cell level and in peripheral blood.
评估了合成代谢类固醇癸酸诺龙（ND）在亚致死剂量照射后增加巨核细胞生成（MP）和造血诱导微环境（HIM）的能力。在接受全身照射（2 Gy）后立即以及接下来的2天，小鼠腹腔注射1.25 mg溶解于丙二醇的ND。受照射的对照小鼠仅接受赋形剂。与对照组相比，药物处理组小鼠在第14天时血小板水平增加了151%。药物处理组小鼠的MP也出现显著反弹，末次注射药物后第8天，骨髓中的集落形成祖干细胞（CFU-Mk）升至对照组的288%，脾脏中的升至599%。此外，药物处理组小鼠在药物处理后第5天，骨髓基质细胞集落增加了286%，脾脏基质细胞集落增加了378%。这些结果表明，ND可增强亚致死剂量照射后的MP和HIM。造血抑制后，ND可能有助于在干细胞水平和外周血中刺激MP和HIM的恢复。
