Xiong Yiyi, Lu Guangyuan, Li Huaixin, He Jianjie, Fan Shipeng, Yan Shuxiang, Zhang Liangxiao, Jia Haibo, Li Maoteng
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoxiong Road, Hongshan District, Wuhan 430074, China.
College of Biology and Food Engineering, Kechuang 1st Road, Maonan District, Guangdong University of Petrochemical Technology, Maoming 525000, China.
Hortic Res. 2024 Jul 24;11(9):uhae196. doi: 10.1093/hr/uhae196. eCollection 2024 Sep.
Sterols are secondary metabolites commonly found in rapeseed that play crucial physiological roles in plants and also benefit human health. Consequently, unraveling the genetic basis of sterol synthesis in rapeseed is highly important. In this study, 21 individual sterols as well as total sterol (TS) content were detected in a double haploid (DH) population of , and a total of 24 quantitative trait loci (QTL) and 157 mQTL were identified that were associated with TS and different individual sterols. Time-series transcriptomic analysis showed that the differentially expressed genes (DEGs) involved in sterol and lipid biosynthesis pathways were enriched. Additionally, a regulatory network between sterol-related DEGs and transcription factors (TFs) was established using coexpression analysis. Some candidate genes were identified with the integration of transcriptomic analysis and QTL mapping, and the key candidate gene . was selected for further functional analysis. demonstrated squalene synthase activity and increased the TS by 3.8% when overexpressed in . The present results provide new insights into sterol regulatory pathways and a valuable genetic basis for breeding rapeseed varieties with high sterol content in the future.
甾醇是油菜籽中常见的次生代谢产物，在植物中发挥着关键的生理作用，对人类健康也有益处。因此，揭示油菜籽中甾醇合成的遗传基础非常重要。在本研究中，在一个双单倍体（DH）群体中检测了21种单个甾醇以及总甾醇（TS）含量，共鉴定出24个数量性状位点（QTL）和157个微QTL，它们与TS和不同的单个甾醇相关。时间序列转录组分析表明，参与甾醇和脂质生物合成途径的差异表达基因（DEG）得到了富集。此外，利用共表达分析建立了甾醇相关DEG与转录因子（TF）之间的调控网络。通过转录组分析和QTL定位的整合鉴定了一些候选基因，并选择关键候选基因进行进一步功能分析。在[具体植物名称]中过表达时，[候选基因名称]表现出鲨烯合酶活性，并使TS增加了3.8%。本研究结果为甾醇调控途径提供了新的见解，并为未来培育高甾醇含量的油菜品种提供了有价值的遗传基础。
