Apaydin Yildirim Betul, Dogan Tuba, Aktas Senocak Esra, Yildirim Serkan, Kordali Saban, Yildirim Fatih
Department of Biochemistry, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Türkiye.
Department of Animal Science, Horasan Vocational College, Atatürk University, Erzurum, Türkiye.
Acta Diabetol. 2025 Apr;62(4):469-480. doi: 10.1007/s00592-024-02371-5. Epub 2024 Sep 11.
Diabetes raises cardiovascular morbidity and mortality worldwide and causes retinopathy, neuropathy, and nephropathy. Punica granatum L. (Pomegranate) is a fruit that has been used for its medicinal properties in various cultures. This article aims to investigate the antioxidant, anti-inflammatory, anti-apoptotic activity of Punica granatum L. peel ethanol extract (PGE) and its efficacy on NF-κB and Nrf-2/HO-1 signaling pathways in kidney tissue of rats with streptozotocin-induced diabetes. Single dose STZ 60 mg/kg/i.p. rats were given to induce diabetes and blood glucose measurements were taken 7 days later. PGE 10 mg/kg/p.o. administered to the treatment groups for 20 days. Blood, kidney, and pancreas samples taken from anesthetized rats were analyzed biochemically and histopathologically. It was observed that STZ increased the levels of urea, uric acid and creatine in the blood, while PGE significantly decreased these parameters. The diabetic group had higher MDA and lower renal tissue GSH level, CAT, GPx, and SOD activity than the control group. STZ also enhanced inflammation, apoptosis, Bax, Caspase-3, and NF-κB expression, and decreased Bcl-2, HO-1, and Nrf-2 expression. Experimental results showed that PGE has the potential to alleviate the harmful effects on the kidney and pancreas by altering the mentioned parameters in diabetic rats.
糖尿病在全球范围内增加了心血管疾病的发病率和死亡率，并引发视网膜病变、神经病变和肾病。石榴（Punica granatum L.）是一种在多种文化中因其药用特性而被使用的水果。本文旨在研究石榴皮乙醇提取物（PGE）的抗氧化、抗炎、抗凋亡活性及其对链脲佐菌素诱导的糖尿病大鼠肾脏组织中NF-κB和Nrf-2/HO-1信号通路的影响。给大鼠腹腔注射单剂量60 mg/kg的链脲佐菌素以诱导糖尿病，并在7天后测量血糖。治疗组口服给予10 mg/kg的PGE，持续20天。对从麻醉大鼠采集的血液、肾脏和胰腺样本进行生化和组织病理学分析。观察到链脲佐菌素增加了血液中尿素、尿酸和肌酐的水平，而PGE显著降低了这些参数。糖尿病组的丙二醛水平高于对照组，肾组织谷胱甘肽水平、过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶活性低于对照组。链脲佐菌素还增强了炎症、细胞凋亡、Bax、Caspase-3和NF-κB的表达，并降低了Bcl-2、HO-1和Nrf-2的表达。实验结果表明，PGE有可能通过改变糖尿病大鼠的上述参数来减轻对肾脏和胰腺的有害影响。
