Engineering Research Center of Low-Carbon Treatment and Green Development of Polluted Water in Northeast China, Ministry of Education, Northeast Normal University, Changchun 130117, PR China; Jilin Engineering Lab for Water Pollution Control and Resources Recovery, Northeast Normal University, Changchun 130117, PR China; Beijing Engineering Research Center of Sustainable Urban Sewage System Construction and Risk Control, Beijing University of Civil Engineering and Architecture, Beijing 100044, PR China.
Engineering Research Center of Low-Carbon Treatment and Green Development of Polluted Water in Northeast China, Ministry of Education, Northeast Normal University, Changchun 130117, PR China; Jilin Engineering Lab for Water Pollution Control and Resources Recovery, Northeast Normal University, Changchun 130117, PR China.
Water Res. 2024 Nov 15;266:122375. doi: 10.1016/j.watres.2024.122375. Epub 2024 Sep 7.
Frequent occurrence of trace antibiotics in reclaimed water is concerning, which inevitably causes aquifer contamination in the case of managed aquifer recharge (MAR). Global governments have formulated strict reclaimed water standards to ensure the safety of water reuse. Recent studies have found that improved antibiotics removal is intimately associated with high ammonia-oxidizing activity. However, the role of NH-N in the removal of residual antibiotics of reclaimed water during MAR remains unknown. NH-N removal and the effects of ammonia oxidation on antibiotics biodegradation in the aquifer are the most significant facts for solving the above collision. In this work, the effects of NH-N (0, 1 and 5 mg/L) in a model refractory antibiotic (oxacillin (OXA), 100 μg/L) attenuation were deciphered by employing three individual simulated MAR columns, which so called N0, N1 and N5. The results showed that 5 mg/L NH-N in influent upregulated the abundance of amo genes by 28.9 %-68.0 % in N5. And the enriched functional genes encoding key degradation enzymes enhanced the OXA removal by 18.7 % and alleviated the oxidative stress caused by antibiotics. Subsequently, antibiotic resistance genes (ARGs), mobile gene elements (MGEs) and human bacterial pathogens (HBPs) abundance were all significantly decreased. Moreover, the intimate association between ammonia-oxidizing microorganisms (AOM) and candidate OXA degraders based on microbial network analysis further supported the significance of AOM on OXA biodegradation. This study provides comprehensive evidence that appropriate amounts of NH-N are beneficial in antibiotics and antibiotic resistance risk reduction, providing compelling insights for refine NH-N recharge limitation.
再生水中痕量抗生素的频繁出现令人担忧，这不可避免地会导致管理型含水层补给（MAR）中的含水层污染。全球各国政府都制定了严格的再生水标准，以确保水再利用的安全。最近的研究发现，提高抗生素去除率与高氨氧化活性密切相关。然而，NH-N 在 MAR 期间去除再生水中残留抗生素的作用尚不清楚。NH-N 的去除以及氨氧化对含水层中抗生素生物降解的影响是解决上述冲突的最重要因素。在这项工作中，通过使用三个单独的模拟 MAR 柱（分别称为 N0、N1 和 N5）来解析 NH-N（0、1 和 5 mg/L）对模型难降解抗生素（100 μg/L 的苯唑西林（OXA））衰减的影响。结果表明，进水 5 mg/L 的 NH-N 将 N5 中 amo 基因的丰度提高了 28.9%-68.0%。富集的功能基因编码关键降解酶，提高了 OXA 的去除率，并减轻了抗生素引起的氧化应激。随后，抗生素耐药基因（ARGs）、移动基因元件（MGEs）和人类细菌病原体（HBPs）的丰度均显著降低。此外，基于微生物网络分析的氨氧化微生物（AOM）与候选 OXA 降解菌之间的密切关联进一步支持了 AOM 对 OXA 生物降解的重要性。本研究提供了全面的证据，表明适量的 NH-N 有利于减少抗生素和抗生素耐药性风险，为细化 NH-N 补给限制提供了有力的见解。
