Yang Shoufeng, Feng Miaomiao, Xu Jianxiong, Deng Zilong, Zhang Hongcai
Shanghai Veterinary Bio-tech Key Laboratory, College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Int J Biol Macromol. 2024 Sep 12;280(Pt 1):135515. doi: 10.1016/j.ijbiomac.2024.135515.
The xylanase and glucose oxidase (GOD) are easily inactivated, restricting their applicaiton in food and agriculture fields. In this work, xylanase and glucose oxidase (GOD) were encapsulated into cellulose nanocrystals (CNC) stabilized three-layer microcapsules via ionic gelation technique to improve their bioavailability and targeted delivery. Encapsulation efficiency (EE), physicochemical properties, and in vitro releasing of xylanase and GOD encapsulated in microcapsules were investigated. EE of xylanase and GOD reached the highest values (73.34 % and 67.16 %, respectively) at an enzyme concentration of 35 mg/mL. In vitro experiments revealed that cumulative release of both enzymes encapsulated in microcapsules was greater than that of controls in simulated gastric tract (SGT) and simulated intestinal tract (SIT). The release of xylanase increased from 41.62 % (gastric tract) to 77.13 % (intestine tract), and release of GOD increased from 42.63 % to 72.11 %, respectively. Novel hydrogel carriers as enzymes encapsulation system could effectively improve the survival rate of enzymes in harsh environments and could be widely employed in food, feed and other industries.
木聚糖酶和葡萄糖氧化酶（GOD）很容易失活，这限制了它们在食品和农业领域的应用。在这项工作中，通过离子凝胶化技术将木聚糖酶和葡萄糖氧化酶（GOD）封装到纤维素纳米晶体（CNC）稳定的三层微胶囊中，以提高它们的生物利用度和靶向递送能力。研究了微胶囊中封装的木聚糖酶和GOD的包封率（EE）、理化性质和体外释放情况。在酶浓度为35 mg/mL时，木聚糖酶和GOD的EE达到最高值（分别为73.34%和67.16%）。体外实验表明，微胶囊中封装的两种酶在模拟胃肠道（SGT）和模拟肠道（SIT）中的累积释放量均大于对照组。木聚糖酶的释放率从41.62%（胃肠道）增加到77.13%（肠道），GOD的释放率分别从42.63%增加到72.11%。新型水凝胶载体作为酶封装系统可以有效提高酶在恶劣环境中的存活率，并可广泛应用于食品、饲料和其他行业。
