Department of Civil Engineering, Indian Institute of Technology, Kharagpur, India.
Department of Civil Engineering, Indian Institute of Technology, Kharagpur, India; Department of Life Sciences, GITAM School of Science, GITAM, Visakhapatnam 530045, Andhra Pradesh, India.
Bioresour Technol. 2024 Dec;413:131467. doi: 10.1016/j.biortech.2024.131467. Epub 2024 Sep 12.
Wastewater resources can be used to produce microbial protein for animal feed or organic fertiliser, conserving food chain resources. This investigation hasemployed thefermented sewage to photoheterotrophically grown purple non-sulfur bacteria (PNSB) in a 2.5 m pilot-scaleraceway-pond with infrared light to produce proteinaceous biomass. Fermented sewage with synthetic media consisting of sodium acetate and propionic acids at a surface-to-volume (S/V) ratio of 10 m/m removed 89%, 93%, and 81% of chemical oxygen demand, ammonium nitrogen, and orthophosphate, respectively; whereas respective removal in fermented sewage alone without synthetic media was 73%, 73%, and 72% during batch operation of 120 h. The biomass yield of 0.88-0.95 g COD /g COD with protein content of 40.3 ± 0.3%-43.9 ± 0.2% w/w was obtained for fermented sewage with synthetic media. The results revealed enhanced possibility of scaling-up the raceway reactor to recover resources from municipal wastewater and enable simultaneous high-rate PNSB single-cell protein production.
污水资源可用于生产动物饲料或有机肥料的微生物蛋白，从而节约食物链资源。本研究采用发酵污水作为碳源，在 2.5m 中试跑道-池塘中，利用红外线对紫色非硫光合细菌（PNSB）进行光异养生长，以生产蛋白质生物质。在表面体积（S/V）比为 10m/m 的乙酸钠和丙酸合成培养基中，发酵污水分别去除了 89%、93%和 81%的化学需氧量、铵态氮和正磷酸盐；而在没有合成培养基的单独发酵污水中，在 120h 的分批运行中，分别去除了 73%、73%和 72%。对于含有合成培养基的发酵污水，生物量产率为 0.88-0.95g COD/g COD，蛋白质含量为 40.3±0.3%-43.9±0.2%w/w。结果表明，从城市污水中回收资源并实现同时高负荷 PNSB 单细胞蛋白生产的跑道式反应器具有较大的放大可能性。
