Department of Veterinary Sciences, University of Messina, Viale Palatucci, 13, Messina, 98168, Italy.
Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, Perugia, 06126, Italy.
Acta Vet Scand. 2024 Sep 11;66(1):47. doi: 10.1186/s13028-024-00767-5.
Centrifugation is a common procedure to improve the quality of chilled and frozen canine semen by removing debris and seminal plasma and adding semen extenders. The aim of this study was to evaluate the efficacy and influence of a second centrifugation after 48 h of storage at 5 °C on the sperm quality of canine semen. The ejaculates of 45 healthy male dogs, divided into three groups according to body weight, were analyzed for macro- and microparameters such as ejaculate volume, sperm concentration, kinematic parameters, morphology, and integrity of plasma membrane. Samples were analyzed at baseline conditions (T), after 24 h (T) and after 48 h (T) to assess the effects of the different treatments on sperm quality.
The results showed a significant effect of a second centrifugation on the improvement of chilled sperm quality compared to the other techniques, especially up to 48 h.
Analysis of the data showed that the semen samples centrifuged and then cooled at 5 °C had acceptable semen parameters, especially in terms of motility, with a gradual decrease in serial evaluations after 24 and 48 h. A second centrifugation after 48 h of storage may lead to better semen quality and improve the kinetics of sperm parameters, the percentage of morphologically normal sperm and the percentage of sperm with intact membranes.
离心是一种常见的程序，通过去除碎片和精液浆并添加精液稀释剂来提高冷藏和冷冻犬精液的质量。本研究的目的是评估在 5°C 储存 48 小时后进行第二次离心对犬精液精子质量的效果和影响。将 45 只健康雄性犬的精液按体重分为三组，分析宏观和微观参数，如精液量、精子浓度、运动参数、形态和质膜完整性。在基线条件（T）、24 小时后（T）和 48 小时后（T）分析样本，以评估不同处理对精子质量的影响。
结果表明，与其他技术相比，第二次离心对冷藏精子质量的改善有显著影响，尤其是在 48 小时内。
数据分析表明，在 5°C 下离心和冷却的精液样本具有可接受的精液参数，尤其是在运动方面，在 24 小时和 48 小时后的连续评估中逐渐下降。在储存 48 小时后进行第二次离心可能会导致更好的精液质量，并改善精子参数的动力学、形态正常精子的百分比和质膜完整精子的百分比。
