Department of Fish Diseases, Faculty of Aquatic Sciences, Istanbul University, Istanbul, Turkiye.
epartment of Fisheries and Aquaculture, Faculty of Agriculture, Ankara University, Ankara, Turkiye.
Cell Mol Biol (Noisy-le-grand). 2024 Sep 8;70(8):32-38. doi: 10.14715/cmb/2024.70.8.4.
Identifying pathogenic microorganisms causing disease is important for epidemiological research, antimicrobial therapy, and control. The current study was carried out to use different methods for the identification of Vibrio anguillarum from diseased rainbow trout (Oncorhynchus mykiss) obtained from Türkiye (Muğla-Fethiye), the damage caused by the pathogenic microorganism in the tissues and organs, and the determination of the antibiotic effective against the pathogen. Hemorrhagic and ulcerative skin lesions and diffuse petechial hemorrhage in the internal organs were clinically detected in diseased fish obtained from the rainbow trout farm. Bacteria isolated from diseased fish were subjected to analysis using conventional bacteriological methods, a commercial bacterial identification test kit (API), an automated bacteria identification system known as Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), BD Phoenix™, and 16S rRNA sequence analysis. All isolated bacteria were identified as V. anguillarum by API 20E and conventional bacteriological method. These results have been confirmed with 16S rRNA sequence analysis. However, the isolated bacteria were identified as Grimontia hollisae (syn. Vibrio holisae) with the BD Phoenix system. Histologically, tissue damage such as melano-macrophage centers and necrosis in the kidney and spleen, hyperemia and mononuclear cell infiltration in the liver, as well as mononuclear cell infiltration on muscles, talengectiasis in the gill tissue was observed. In addition, it has been determined that the most effective antibiotic against the pathogen was enrofloxacin. When comparing all identification methods used for this pathogen causing tissue damage, it was demonstrated that the MALDI-TOF MS provides better results than other methods in terms of cost and identification time, and it could be used as an alternative to the conventional method to the identification of V. anguillarum.
鉴定引起疾病的致病微生物对于流行病学研究、抗菌治疗和控制非常重要。本研究旨在使用不同方法从土耳其穆格拉-费特希耶的患病虹鳟鱼（Oncorhynchus mykiss）中鉴定鳗弧菌，确定致病微生物对组织和器官的损伤，并确定对抗病原体有效的抗生素。从虹鳟养殖场获得的患病鱼中临床检测到出血和溃疡皮肤病变以及内部器官弥漫性瘀点出血。从患病鱼中分离的细菌通过常规细菌学方法、商业细菌鉴定试剂盒（API）、称为基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）的自动细菌鉴定系统、BD Phoenix™和 16S rRNA 序列分析进行分析。所有分离的细菌均通过 API 20E 和常规细菌学方法鉴定为鳗弧菌。这些结果已通过 16S rRNA 序列分析得到证实。然而，BD Phoenix 系统将分离的细菌鉴定为 Grimontia hollisae（ synonym Vibrio holisae）。组织学上，观察到肾脏和脾脏中的黑色素巨噬细胞中心和坏死、肝脏中的充血和单核细胞浸润以及肌肉上的单核细胞浸润、鳃组织中的 talengectiasis。此外，已确定对抗病原体最有效的抗生素是恩诺沙星。在比较所有用于鉴定这种引起组织损伤的病原体的方法时，结果表明 MALDI-TOF MS 在成本和鉴定时间方面优于其他方法，并且可以替代传统方法用于鉴定鳗弧菌。
