Lee Hak-Dong, Kim Ji Hyun, Choi Jong Hee, Kim Ki Hyun, Ku Jajung, Choi Kyung, Kim Hyun Young, Lee Sanghyun, Cho Ik-Hyun
Department of Plant Science and Technology, Chung-Ang University, Anseong, Republic of Korea.
Natural Product Institute of Science and Technology, Anseong, Republic of Korea.
Front Pharmacol. 2024 Aug 28;15:1406623. doi: 10.3389/fphar.2024.1406623. eCollection 2024.
(PT), a tree endemic to Korea, shows promising potential as a natural therapeutic agent owing to its potent anti-inflammatory properties. However, the isolation and analysis of phytochemical compounds in PT and related species remains underexplored. Therefore, this study aims to investigate the biochemical profile of PT and evaluate its extracts and fractions for anti-inflammatory activities. Nine compounds were isolated, including two novel flavonoids (luteolin 7-O--d-glucuronide butyl ester and chrysoeriol 7-O--d-glucuronide butyl ester) from the Salicaceae family for the first time. The ethyl acetate fraction exhibited significant radical scavenging activity against various radicals, including DPPH, ABTS, OH, and O radicals. PT extracts and the ethyl acetate fraction showed minimal cytotoxicity in Raw 264.7 macrophages at concentrations below 500 and 100 μg/mL, respectively. Furthermore, PT extracts and fractions significantly suppressed the protein expression of proinflammatory mediators (iNOS and IL-6) in LPS-stimulated Raw 264.7 macrophages, highlighting their potent anti-inflammatory effects. These findings suggest that PT holds promise as a valuable natural therapeutic intervention for various oxidative stress and inflammation-related disorders, underscoring the need for further exploration of its clinical applications.
紫椴（PT）是韩国特有的一种树，因其强大的抗炎特性，作为一种天然治疗剂显示出有前景的潜力。然而，紫椴及其相关物种中植物化学化合物的分离和分析仍未得到充分探索。因此，本研究旨在研究紫椴的生化特征，并评估其提取物和馏分的抗炎活性。首次从杨柳科分离出九种化合物，包括两种新型黄酮类化合物（木犀草素7 - O - β - D - 葡萄糖醛酸丁酯和 Chrysoeriol 7 - O - β - D - 葡萄糖醛酸丁酯）。乙酸乙酯馏分对包括DPPH、ABTS、OH和O自由基在内的各种自由基表现出显著的自由基清除活性。紫椴提取物和乙酸乙酯馏分在浓度分别低于500和100μg/mL时，在Raw 264.7巨噬细胞中显示出最小的细胞毒性。此外，紫椴提取物和馏分显著抑制LPS刺激的Raw 264.7巨噬细胞中促炎介质（iNOS和IL - 6）的蛋白表达，突出了它们强大的抗炎作用。这些发现表明，紫椴有望成为治疗各种氧化应激和炎症相关疾病的有价值的天然治疗干预手段，强调了进一步探索其临床应用的必要性。
