Shi Yiwan, Wang Zhaozhen, Xu Weikang, Yu Xiaolu, Gao Botao, Zhou Xinting, Chen Jiwen, Jia Kunfeng, Cheang Lek Hang, Tam Man Seng, Wang Huajun, Zheng Xiaofei, Wu Tingting
National Engineering Research Center for Healthcare Devices, Guangdong Provincial Key Laboratory of Medical Electronic Instruments and Materials, Institute of Biological And Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China.
Department of Sports Medicine, The First Affiliated Hospital, Guangdong Provincial Key Laboratory of Speed Capability, The Guangzhou Key Laboratory of Precision Orthopedics and Regenerative Medicine, Jinan University, Guangzhou, PR China.
J Biomater Appl. 2025 Jan;39(6):578-591. doi: 10.1177/08853282241281439. Epub 2024 Sep 12.
Large bone defect repair is a striking challenge in orthopedics. Currently, inorganic-organic composite scaffolds are considered as a promising approach to these bone regeneration. Silicon ions (Si) are bioactive and beneficial to bone regeneration and Si-containing inorganic mesoporous silica (MS) can effectively load drugs for bone repair. To better control the release of drug, we prepared biodegradable MS/PLGA (MP) microspheres. MP loaded organic silk fibroin/carboxymethyl chitosan/sodium alginate (MP/SF/CMCS/SA) composite scaffolds were further constructed by genipin and Ca crosslinking. All MP/SF/CMCS/SA scaffolds had good swelling ability, degradation rate and high porosity. The incorporation of 1% MP significantly enhanced the compressive strength of composite scaffolds. Besides, MP loaded scaffold showed a sustained release of Si and Ca. Moreover, the release rate of rhodamine (a model drug) of MP/SF/CMCS/SA scaffolds was obviously lower than that of MP. When culturing with rat bone marrow mesenchymal stem cells, scaffolds with 1% MP displayed good proliferation, adhesion and enhanced osteogenic differentiation ability. Based on the results above, the addition of 1% MP in SF/CMCS/SA scaffolds is a prospective way for drug release in bone regeneration and is promising for further in vivo bone repair applications.
大骨缺损修复是骨科领域一项极具挑战性的工作。目前，无机 - 有机复合支架被认为是实现这些骨再生的一种有前景的方法。硅离子（Si）具有生物活性且对骨再生有益，含硅的无机介孔二氧化硅（MS）能够有效负载用于骨修复的药物。为了更好地控制药物释放，我们制备了可生物降解的MS/PLGA（MP）微球。通过京尼平与钙离子交联进一步构建了负载MP的有机丝素蛋白/羧甲基壳聚糖/海藻酸钠（MP/SF/CMCS/SA）复合支架。所有MP/SF/CMCS/SA支架都具有良好的溶胀能力、降解速率和高孔隙率。加入1%的MP显著提高了复合支架的抗压强度。此外，负载MP的支架显示出硅和钙的持续释放。而且，MP/SF/CMCS/SA支架中罗丹明（一种模型药物）的释放速率明显低于MP。当与大鼠骨髓间充质干细胞共培养时，含有1%MP的支架表现出良好的增殖、黏附能力以及增强的成骨分化能力。基于上述结果，在SF/CMCS/SA支架中添加1%的MP是骨再生中药物释放的一种有前景的方式，有望进一步应用于体内骨修复。
