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转录因子 RUNT 样通过促进蛹期表皮蛋白转录来调控蛹期表皮发育。

The transcription factor RUNT-like regulates pupal cuticle development via promoting a pupal cuticle protein transcription.

机构信息

Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China.

出版信息

PLoS Genet. 2024 Sep 12;20(9):e1011393. doi: 10.1371/journal.pgen.1011393. eCollection 2024 Sep.

DOI:10.1371/journal.pgen.1011393
PMID:39264939
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11392391/
Abstract

Holometabolous insects undergo morphological remodeling from larvae to pupae and to adults with typical changes in the cuticle; however, the mechanism is unclear. Using the lepidopteran agricultural insect Helicoverpa armigera, cotton bollworm, as a model, we revealed that the transcription factor RUNT-like (encoded by Runt-like) regulates the development of the pupal cuticle via promoting a pupal cuticle protein gene (HaPcp) expression. The HaPcp was highly expressed in the epidermis and wing during metamorphosis and was found being involved in pupal cuticle development by RNA interference (RNAi) analysis in larvae. Runt-like was also strongly upregulated in the epidermis and wing during metamorphosis. Knockdown of Runt-like produced similar phenomena, a failure of abdomen yellow envelope and wing formation, to those following HaPcp knockdown. The insect molting hormone 20-hydroxyecdysonen (20E) upregulated HaPcp transcription via RUNT-like. 20E upregulated Runt-like transcription via nuclear receptor EcR and the transcription factor FOXO. Together, RUNT-like and HaPCP are involved in pupal cuticle development during metamorphosis under 20E regulation.

摘要

完全变态的昆虫在从幼虫到蛹再到成虫的过程中经历形态重塑，其外骨骼会发生典型变化；然而，其机制尚不清楚。本研究以鳞翅目农业害虫棉铃虫为模型，揭示了转录因子 Runt-like（由 Runt-like 编码）通过促进蛹皮蛋白基因（HaPcp）的表达来调节蛹皮的发育。HaPcp 在变态过程中在外皮和翅膀中高度表达，通过在幼虫中进行 RNA 干扰（RNAi）分析发现其参与蛹皮发育。Runt-like 在变态过程中外皮和翅膀中强烈上调。Runt-like 敲低产生了与 HaPcp 敲低相似的现象，即腹部黄囊和翅膀形成失败。蜕皮激素 20-羟基蜕皮酮（20E）通过 Runt-like 上调 HaPcp 的转录。20E 通过核受体 EcR 和转录因子 FOXO 上调 Runt-like 的转录。总之，RUNT-like 和 HaPCP 参与了 20E 调控下的变态过程中的蛹皮发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/19a295dd9ded/pgen.1011393.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/4dc6913e5f38/pgen.1011393.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/f9901f53608e/pgen.1011393.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/0197f12c5214/pgen.1011393.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/c1d2917bf02f/pgen.1011393.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/cbbce89958f0/pgen.1011393.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/b2581a1f5845/pgen.1011393.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/ac008abb2578/pgen.1011393.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/656e383923c3/pgen.1011393.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/19a295dd9ded/pgen.1011393.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/4dc6913e5f38/pgen.1011393.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/f9901f53608e/pgen.1011393.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/0197f12c5214/pgen.1011393.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/c1d2917bf02f/pgen.1011393.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/cbbce89958f0/pgen.1011393.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/b2581a1f5845/pgen.1011393.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/ac008abb2578/pgen.1011393.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/656e383923c3/pgen.1011393.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/680f/11392391/19a295dd9ded/pgen.1011393.g009.jpg

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