Stöffler-Meilicke M, Dabbs E R, Albrecht-Ehrlich R, Stöffler G
Eur J Biochem. 1985 Aug 1;150(3):485-90. doi: 10.1111/j.1432-1033.1985.tb09048.x.
A mutant of Escherichia coli has been isolated which lacked ribosomal proteins S17 and L29, as judged by two-dimensional gel electrophoresis. A battery of immunological tests was used to confirm this result. Ribosomes of this mutant were used as a control for the localization of proteins S17 and L29 on the surface of the ribosomal subunits of E. coli. Protein S17 has been localized on the 30S subunit body, 3-5 nm away from the lower pole, while protein L29 is located at the back of the 50S particle on the opposite side to the interface.
通过二维凝胶电泳判断，已分离出一种缺乏核糖体蛋白S17和L29的大肠杆菌突变体。使用一系列免疫学检测来证实这一结果。该突变体的核糖体被用作对照，以确定蛋白S17和L29在大肠杆菌核糖体亚基表面的定位。蛋白S17已定位在30S亚基主体上，距离下极3 - 5纳米，而蛋白L29位于50S颗粒与界面相对一侧的背面。
