脂肪源性基质血管成分对环磷酰胺诱导的卵巢损伤大鼠卵巢功能的保护作用

The Protective Effect of Adipose-Derived Stromal Vascular Fraction on Ovarian Function in Rats with Cyclophosphamide-Induced Ovarian Damage.

作者信息

Okten Sabri Berkem, Ozcan Pinar, Tok Olgu Enis, Devranoglu Belgin, Cetin Caglar, Tanoglu Fatma Basak, Ficicioglu Cem

机构信息

Acibadem Health Group, Acibadem Kozyatagi Hospital, Department of Obstetrics, Gynecology and Reproductive Medicine, İstanbul, Turkey.

Department of Obstetrics and Gynecology, Faculty of Medicine, Bezmialem University, İstanbul, Turkey.

出版信息

Gynecol Obstet Invest. 2025;90(2):120-128. doi: 10.1159/000541049. Epub 2024 Sep 12.

DOI:10.1159/000541049

PMID:39265557

Abstract

OBJECTIVE

The aim of this study was to investigate if adipose-derived stromal vascular fraction (SVF) treatment has any protective effect on ovarian function in rats with cyclophosphamide (CP) induced ovarian damage.

DESIGN

This was an experimental animal study.

PARTICIPANTS/MATERIALS, SETTING, METHODS: 25 mature cycling Wistar-Albino rats were randomized into four groups (n = 5 per group). Rats in groups 1 and 2 received single dose of intraperitoneal (i.p.) 1 mL/kg sodium chloride 0.9% (NaCl). Groups 3 and 4 received single dose of 75 mg/kg i.p. CP. On seventh day, SVF was prepared from adipose tissues of 5 additional rats and groups 1 and 3 received 0.9% NaCl i.p. injections while groups 2 and 4 received 0.2 mL i.p. injections of SVF. On day 21 all rats were euthanized, and serum anti-mullerian hormone (AMH) levels, primordial, primary, secondary, antral, and atretic follicle counts, AMH positive staining follicle counts along with AMH staining intensity of the follicles were evaluated.

RESULTS

Among two CP induced ovarian damaged groups, SVF treated group showed significantly higher secondary and antral follicle and lower atretic follicle counts, significantly higher mean serum AMH levels, AMH positive antral follicle count and higher intensity of AMH positive follicle scores for primary, secondary, and antral follicles when compared to untreated group. Moreover, group 1 showed no significant difference for all parameters except antral follicle count and AMH positive staining intensity scores for antral follicles when compared to group 4.

LIMITATIONS

This study was conducted on experimental rat model.

CONCLUSION

Our study demonstrated a significant protective effect of SVF against CP-induced ovarian damage which reveals the apparent need for further investigation of its precise mechanisms of action as it may provide a new treatment approach for women with premature ovarian failure.

摘要

目的

本研究旨在探讨脂肪来源的基质血管成分（SVF）治疗对环磷酰胺（CP）诱导的卵巢损伤大鼠的卵巢功能是否具有保护作用。

设计

这是一项实验动物研究。

参与者/材料、设置、方法：25只成熟的性周期正常的Wistar白化大鼠被随机分为四组（每组n = 5）。第1组和第2组大鼠腹腔注射（i.p.）单剂量1 mL/kg 0.9%氯化钠（NaCl）。第3组和第4组大鼠腹腔注射单剂量75 mg/kg CP。在第7天，从另外5只大鼠的脂肪组织中制备SVF，第1组和第3组腹腔注射0.9% NaCl，而第2组和第4组腹腔注射0.2 mL SVF。在第21天，所有大鼠被安乐死，并评估血清抗苗勒管激素（AMH）水平、原始卵泡、初级卵泡、次级卵泡、窦状卵泡和闭锁卵泡计数、AMH阳性染色卵泡计数以及卵泡的AMH染色强度。

结果

在两个CP诱导的卵巢损伤组中，与未治疗组相比，SVF治疗组的次级卵泡和窦状卵泡计数显著更高，闭锁卵泡计数更低，平均血清AMH水平显著更高，AMH阳性窦状卵泡计数以及初级、次级和窦状卵泡的AMH阳性卵泡评分强度更高。此外，与第4组相比，第1组除窦状卵泡计数和窦状卵泡的AMH阳性染色强度评分外，所有参数均无显著差异。