Sichuan Academy of Grassland Science, Chengdu, 610097, China.
College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
BMC Plant Biol. 2024 Sep 13;24(1):857. doi: 10.1186/s12870-024-05564-w.
Reed canary grass has been identified as a suitable species for restoring plateau wetlands and understanding plant adaptation mechanisms in wetland environments. In this study, we subjected a reed canary grass cultivar 'Chuanxi' to waterlogging, salt, and combined stresses to investigate its phenotypic characteristics, physiological indices, and transcriptome changes under these conditions.
The results revealed that the growth rate was slower under salt stress than under waterlogging stress. The chlorophyll content and energy capture efficiency of the PS II reaction center decreased with prolonged exposure to each stress. Conversely, while the activities of enzymes associated with respiratory metabolism, as well as MDA, PRO, Na, and K-ATPase, increased. The formation of distinct aerenchyma was observed under waterlogging stress and combined stress. Transcriptome sequencing analysis identified 5,379, 4,169, and 14,993 DEGs under CK vs. W, CK vs. S, and CK vs. SW conditions, respectively. The WRKY was found to be the most abundant under waterlogging stress, whereas the MYB predominated under salt stress and combined stress. Glutathione metabolic pathways and Plant hormone signal transduction have also been found to play important roles in stress.
By integrating phenotypic, physiological, anatomical, and transcriptomic, this research provides valuable insights into how reed canary grass responds to salt, waterlogging, and combined stresses. These findings may inform the ecological application of reed canary grass in high-altitude wetlands and for breeding purposes.
芦竹已被确定为恢复高原湿地和了解湿地环境中植物适应机制的适宜物种。本研究以芦竹品种‘川系’为材料，进行水淹、盐和复合胁迫处理，研究其在胁迫条件下的表型特征、生理指标和转录组变化。
盐胁迫下的生长速率慢于水淹胁迫。叶绿素含量和 PS II 反应中心的能量捕获效率随着每种胁迫的持续时间的延长而降低。相反，与呼吸代谢相关的酶的活性以及 MDA、PRO、Na 和 K-ATPase 的活性增加。在水淹胁迫和复合胁迫下观察到明显的通气组织形成。转录组测序分析表明，在 CK 与 W、CK 与 S 和 CK 与 SW 条件下分别有 5379、4169 和 14993 个 DEGs。在水淹胁迫下 WRKY 基因家族最为丰富，而在盐胁迫和复合胁迫下 MYB 基因家族最为丰富。谷胱甘肽代谢途径和植物激素信号转导也被发现对胁迫有重要作用。
通过整合表型、生理、解剖和转录组学研究，本研究深入了解了芦竹对盐、水淹和复合胁迫的响应机制。这些发现可能为芦竹在高海拔湿地的生态应用和育种提供参考。
