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Vetscan Imagyst在犬类粪便样本即时检测中的性能。

Performance of the Vetscan Imagyst in point-of-care detection of in canine fecal samples.

作者信息

Kanski Sabrina, Busch Kathrin, Hailmann Regina, Weber Karin

机构信息

Small Animal Clinic, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany.

出版信息

J Vet Diagn Invest. 2025 Jan;37(1):63-70. doi: 10.1177/10406387241279177. Epub 2024 Sep 12.

Abstract

is a common parasite of the gastrointestinal tract of dogs, with an especially high prevalence in dogs <1-y-old. Methods for detecting are point-of-care (POC) tests such as lateral-flow tests or fecal flotation. The Vetscan Imagyst (Zoetis) is a new POC device for the detection of in fecal samples using zinc sulfate flotation, automated slide scanning, and image recognition with artificial intelligence. Vetscan results are the number of cysts per coverslip. We compared the performance of the Vetscan and another POC test (SNAP test; Idexx) with a direct immunofluorescence assay (IFA) performed in a specialized parasitology laboratory as the reference test. We included 164 dogs <19-mo-old. We used pooled fecal samples from 3 defecations gained within 2-3 d and tested the repeatability of the Vetscan by triplicate measurement. Compared to IFA, Vetscan had a diagnostic sensitivity of 88.4% and specificity of 98.1%; SNAP had a diagnostic sensitivity of 74.4% and specificity of 98.1%. A variation coefficient of 67.0% was determined for the Vetscan results. The performance of the Vetscan is acceptable for the qualitative evaluation of fecal samples ( positive or negative), and the device can be used by untrained personnel. Given its high variation coefficient, we do not recommend the Vetscan for monitoring the number of cysts.

摘要

是犬胃肠道常见寄生虫,在1岁以下犬中患病率尤其高。检测方法为即时检验(POC),如侧向流动检测或粪便漂浮法。Vetscan Imagyst(硕腾公司)是一种新型POC设备,用于通过硫酸锌漂浮、自动载玻片扫描和人工智能图像识别来检测粪便样本中的 。Vetscan的结果是每个盖玻片上 囊肿的数量。我们将Vetscan和另一种POC检测(SNAP检测;爱德士公司)的性能与在专门寄生虫学实验室进行的直接免疫荧光测定(IFA)作为参考检测进行了比较。我们纳入了164只19个月龄以下的犬。我们使用了在2 - 3天内收集的3次排便的混合粪便样本,并通过一式三份测量来测试Vetscan的重复性。与IFA相比,Vetscan的诊断敏感性为88.4%,特异性为98.1%;SNAP的诊断敏感性为74.4%,特异性为98.1%。Vetscan结果的变异系数为67.0%。Vetscan的性能对于粪便样本的定性评估(阳性或阴性)是可接受的,并且该设备未经培训的人员也可使用。鉴于其高变异系数,我们不建议使用Vetscan监测囊肿数量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bbf/11559838/7a9fa2fa5d7e/10.1177_10406387241279177-fig1.jpg

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