Valencia R, N'Guyen Công H, Bertaux O
J Chromatogr. 1985 May 29;325(1):207-20. doi: 10.1016/s0021-9673(00)96021-7.
Separation of the major bases of DNA along with seven minor methylated ones was obtained by reversed-phase high-performance liquid chromatography using an isocratic elution system. Application of this procedure to DNA treated in vitro by N-methyl-N-nitrosourea has allowed identification of two induced minor bases; a third one was resolved using a slightly modified mobile phase. Baseline resolution of 3-methyl- and 5-methylcytosine, detected in Euglena DNA hydrolyzates, was also achieved.
采用等度洗脱系统的反相高效液相色谱法实现了DNA主要碱基与7种次要甲基化碱基的分离。将该方法应用于经N-甲基-N-亚硝基脲体外处理的DNA，已鉴定出两种诱导产生的次要碱基；使用略有改良的流动相分离出了第三种。在眼虫DNA水解产物中检测到的3-甲基胞嘧啶和5-甲基胞嘧啶也实现了基线分离。
