Chronobiology Laboratory, School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695551, India.
G3 (Bethesda). 2024 Nov 6;14(11). doi: 10.1093/g3journal/jkae220.
The biological rhythms generated by the endogenous circadian clocks across the tree of life regulate numerous behavioral, metabolic, and physiological processes. Although evidence from various studies in Drosophila melanogaster indicates the importance of the core circadian clock genes in the intricate interplay between the circadian clock and metabolism, little is known about the contribution of the circadian photoreceptor/s in this process. The deep brain circadian photoreceptor CRYPTOCHROME (CRY) is essential for resetting the clock in response to light and is also highly expressed in metabolically active tissues in Drosophila. In this study, we sought to explore the possible roles played by CRY in triglyceride (TG) metabolism. We observed that the cry mutant (cry01) flies exhibited increased starvation resistance and TG levels under both 12-hour (h) light:12-h dark cycle (LD) and under constant light compared with the control w1118 flies. We also observed that cry01 flies had significantly increased food intake, glycogen concentrations, and lifespan under LD. In addition, cryptochrome seemed to affect TG levels in adult flies in response to calorie-restricted and high-fat diets. These results suggest a role for the circadian photoreceptor CRY in TG metabolism in Drosophila.
生物节律由生命之树中内源性的生物钟产生，调节着众多行为、代谢和生理过程。尽管来自黑腹果蝇的各种研究证据表明核心生物钟基因在生物钟和代谢之间的复杂相互作用中很重要，但对于生物钟光感受器在这个过程中的贡献知之甚少。深脑生物钟光感受器隐花色素（CRY）对于响应光重置时钟是必不可少的，并且在果蝇中代谢活跃的组织中高度表达。在这项研究中，我们试图探索 CRY 在甘油三酯（TG）代谢中可能发挥的作用。我们观察到，cry 突变体（cry01）苍蝇在 12 小时光照：12 小时黑暗周期（LD）和持续光照下比对照 w1118 苍蝇表现出更强的饥饿抗性和 TG 水平。我们还观察到，cry01 苍蝇在 LD 下的食物摄入量、糖原浓度和寿命显著增加。此外，隐花色素似乎会影响成年苍蝇对限食和高脂肪饮食的 TG 水平。这些结果表明，生物钟光感受器 CRY 在果蝇的 TG 代谢中起作用。
