Yu Shifeng, Dai Wenting, Su Chao, Milosavic Nenad, Wang Ziran, Wang Xuejun, Zhu Yibo, He Maogang, Landry Donald W, Stojanovic Milan N, Lin Qiao
College of Mechanical and Vehicle Engineering, Chongqing University, Chongqing 400044, China.
Department of Mechanical Engineering, Columbia University, New York, New York 10027, United States.
ACS Sens. 2024 Sep 27;9(9):4915-4923. doi: 10.1021/acssensors.4c01519. Epub 2024 Sep 13.
This paper presents an aptameric graphene nanosensor for rapid and sensitive measurement of arginine vasopressin (AVP) toward continuous monitoring of critical care patients. The nanosensor is a field-effect transistor (FET) with monolayer graphene as the conducting channel and is functionalized with a new custom-designed aptamer for specific AVP recognition. Binding between the aptamer and AVP induces a change in the carrier density in the graphene and resulting in measurable changes in FET characteristics for determination of the AVP concentration. The aptamer, based on the natural enantiomer D-deoxyribose, possess optimized kinetic binding properties and is attached at an internal position to the graphene for enhanced sensitivity to low concentrations of AVP. Experimental results show that this aptameric graphene nanosensor is highly sensitive (with a limit of detection of 0.3 pM and a resolution of 0.1 pM) to AVP, and rapidly responsive (within 90 s) to both increasing and decreasing AVP concentration changes. The device is also reversable (within 4%), repeatable (within 4%) and reproducible (within 5%) in AVP measurements.
本文介绍了一种适体石墨烯纳米传感器，用于快速、灵敏地测量精氨酸加压素（AVP），以持续监测重症监护患者。该纳米传感器是一种以单层石墨烯为导电通道的场效应晶体管（FET），并用一种新的定制设计适体进行功能化，以实现对AVP的特异性识别。适体与AVP之间的结合会引起石墨烯中载流子密度的变化，从而导致FET特性发生可测量的变化，用于测定AVP浓度。该适体基于天然对映体D-脱氧核糖，具有优化的动力学结合特性，并连接在石墨烯的内部位置，以增强对低浓度AVP的敏感性。实验结果表明，这种适体石墨烯纳米传感器对AVP高度敏感（检测限为0.3 pM，分辨率为0.1 pM），对AVP浓度的增加和减少变化均能快速响应（90秒内）。该器件在AVP测量中还具有可逆性（4%以内）、可重复性（4%以内）和可再现性（5%以内）。
